Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Division of Polymer Chemistry, Department of Chemistry-Ångstrom Laboratory, Uppsala University, Uppsala 75121, Sweden.
Biomed Mater. 2023 Jul 25;18(5). doi: 10.1088/1748-605X/ace0ec.
Biological materials such as cell-derived membrane vesicles have emerged as alternative sources for molecular delivery systems, owing to multicomponent features, the inherent functionalities and signaling networks, and easy-to-carry therapeutic agents with various properties. Herein, red blood cell membrane (RBCM) vesicle-laden methacrylate kappa-carrageenan (KaMA) composite hydrogel is introduced for soft tissue engineering. Results revealed that the characteristics of hybrid hydrogels were significantly modulated by changing the RBCM vesicle content. For instance, the incorporation of 20% (v/v) RBCM significantly enhanced compressive strength from 103 ± 26 kPa to 257 ± 18 kPa and improved toughness under the cyclic loading from 1.0 ± 0.4 kJ mto 4.0 ± 0.5 kJ mafter the 5cycle. RBCM vesicles were also used for the encapsulation of curcumin (CUR) as a hydrophobic drug molecule. Results showed a controlled release of CUR over three days of immersion in PBS solution. The RBCM vesicles laden KaMA hydrogels also supportedfibroblast cell growth and proliferation. In summary, this research sheds light on KaMA/RBCM hydrogels, that could reveal fine-tuned properties and hydrophobic drug release in a controlled manner.
生物材料，如细胞衍生的膜囊泡，已成为分子递药系统的替代来源，这归因于其多组分特性、固有功能和信号网络，以及易于携带具有各种性质的治疗剂。在此，我们介绍了负载红细胞膜囊泡的甲基丙烯酰化 κ-卡拉胶（KaMA）复合水凝胶，用于软组织工程。结果表明，通过改变 RBCM 囊泡含量可以显著调节杂化水凝胶的特性。例如，掺入 20%（体积比）的 RBCM 可以显著提高压缩强度，从 103±26 kPa 提高到 257±18 kPa，并且在 5 个循环后，在循环加载下的韧性从 1.0±0.4 kJ m提高到 4.0±0.5 kJ m。RBCM 囊泡也用于包封姜黄素（CUR）作为疏水性药物分子。结果表明，在 PBS 溶液中浸泡三天可以实现 CUR 的控制释放。负载 RBCM 囊泡的 KaMA 水凝胶还支持成纤维细胞的生长和增殖。总之，这项研究揭示了 KaMA/RBCM 水凝胶，其可以在控制的方式下展示精细调节的特性和疏水性药物释放。
