Zhang Meihua, Zheng Amy, Zheng Zhongquan C, Wang Michael Zhuo
1 Department of Aerospace Engineering, The University of Kansas, Lawrence, KS, USA.
2 Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA.
Proc Inst Mech Eng H. 2019 Apr;233(4):432-443. doi: 10.1177/0954411919838715.
A microfluidic-based microscale cell-culture device, or a cells-on-a-chip device, provides a well-controlled environment with physiologically realistic factors that emulate the organ-to-organ network of human body. In the microsystem, the in vivo situation can be resembled closely by controlling the chip geometry model, medium flow behavior, medium-to-cell density ratio, and other fluid dynamic parameters. This study is to develop multiphase models to carry out experiments and simulate flow in such devices. A standard soft lithography method is used to build the three-dimensional microfluidic chips. A definitely good qualitative and reasonably good quantitative agreement is obtained between the experimental and simulation results for particle velocity in the microfluidic chip, which validates the numerical simulation method. The cell deposition rate influenced by the flow shear is studied. The influence of gravity, inlet velocity, and cell injection number on cell concentrations are also investigated. Comparisons of different designs of cells-on-a-chip devices are addressed in the study. The physics of flow dynamics and related cell particle motion due to each of the above-mentioned variables are discussed. The results show that the multiphase flow model is promising to be used for simulating cell particle deposition and concentration for the purpose of design of cells-on-a-chip devices.
基于微流控的微尺度细胞培养装置，即芯片上的细胞装置，提供了一个具有生理现实因素的良好控制环境，可模拟人体器官间网络。在该微系统中，通过控制芯片几何模型、介质流动行为、介质与细胞密度比及其他流体动力学参数，可非常接近地模拟体内情况。本研究旨在开发多相模型，以在此类装置中进行实验并模拟流动。采用标准软光刻方法构建三维微流控芯片。在微流控芯片中，实验结果与粒子速度模拟结果之间获得了明确良好的定性和合理良好的定量一致性，验证了数值模拟方法。研究了流动剪切对细胞沉积速率的影响。还研究了重力、入口速度和细胞注入数量对细胞浓度的影响。本研究探讨了芯片上细胞装置不同设计的比较。讨论了上述每个变量引起的流动动力学物理和相关细胞粒子运动。结果表明，多相流模型有望用于模拟细胞粒子沉积和浓度，以用于芯片上细胞装置的设计。
