Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, 48109, USA.
Nat Commun. 2019 Apr 1;10(1):1478. doi: 10.1038/s41467-019-09439-9.
Circulating tumor cells (CTCs) have become an established biomarker for prognosis in patients with various carcinomas. However, current ex vivo CTC isolation technologies rely on small blood volumes from a single venipuncture limiting the number of captured CTCs. This produces statistical variability and inaccurate reflection of tumor cell heterogeneity. Here, we describe an in vivo indwelling intravascular aphaeretic CTC isolation system to continuously collect CTCs directly from a peripheral vein. The system returns the remaining blood products after CTC enrichment, permitting interrogation of larger blood volumes than classic phlebotomy specimens over a prolonged period of time. The system is validated in canine models showing capability to screen 1-2% of the entire blood over 2 h. Our result shows substantial increase in CTC capture, compared with serial blood draws. This technology could potentially be used to analyze large number of CTCs to facilitate translation of analytical information into future clinical decisions.
循环肿瘤细胞 (CTCs) 已成为各种癌预后的既定生物标志物。然而，目前的体外 CTC 分离技术依赖于单次静脉穿刺的少量血液，限制了捕获的 CTC 数量。这会产生统计变异性，并不能准确反映肿瘤细胞的异质性。在这里，我们描述了一种体内留置血管内吸咐 CTC 分离系统，可从外周静脉连续采集 CTC。该系统在 CTC 富集后会回输剩余的血液制品，从而允许在较长时间内对比经典采血标本更大体积的血液进行检测。该系统在犬模型中得到了验证，能够在 2 小时内筛选出 1-2%的全血。与连续采血相比，我们的结果显示 CTC 的捕获量有了实质性的增加。这项技术有可能被用于分析大量的 CTC，以促进将分析信息转化为未来的临床决策。
