Lu Yanyan, Chang Xiaoxiao, Guo Xinbo
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510641, China.
Plants (Basel). 2019 Mar 25;8(3):75. doi: 10.3390/plants8030075.
To better understand the regulatory mechanism of phenolics and ascorbic acid accumulation as well as antioxidant activities in mung beans during legume development, the gene expression profiles of 25 key-coding genes in ascorbic acid and phenolics metabolic pathways were analyzed. As well as the dynamitic changes of ascorbic acid, phenolic profiles and antioxidant activities with legume development were studied. The results indicated that gene expression profiles were closely related to the ascorbic acid and phenolics accumulation regularity during legume development. and played important roles for ascorbic acid accumulation from 8 to 17 days after flowering (DAF). and exhibited positive correlations with daidzein and glycitin accumulation, and had a strong positive correlation with glycitin biosynthesis. Antioxidant activities dramatically increased during mung bean maturing, which were significantly related to ascorbic acid and phenolics accumulation. Eight days after flowering was the essential stage for ascorbic acid and phenolics biosynthesis in mung beans.
为了更好地理解豆类发育过程中绿豆中酚类和抗坏血酸积累的调控机制以及抗氧化活性，分析了抗坏血酸和酚类代谢途径中25个关键编码基因的基因表达谱。同时研究了随着豆类发育抗坏血酸、酚类物质组成及抗氧化活性的动态变化。结果表明，基因表达谱与豆类发育过程中抗坏血酸和酚类物质的积累规律密切相关。基因1和基因2在开花后8至17天（DAF）对抗坏血酸积累起重要作用。基因3和基因4与黄豆苷元和大豆黄素积累呈正相关，基因5与大豆黄素生物合成呈强正相关。绿豆成熟过程中抗氧化活性显著增加，这与抗坏血酸和酚类物质的积累显著相关。开花后8天是绿豆中抗坏血酸和酚类物质生物合成的关键阶段。
