Fischer A M, Yu X J, Tapon-Bretaudiere J, Muller D, Bros A, Jozefonvicz J
J Chromatogr. 1986 Aug 8;363(1):95-100. doi: 10.1016/s0021-9673(00)88994-3.
Insoluble polystyrenes substituted with sulphonate and L-arginyl methyl ester have been synthesized. Using their specific affinity for thrombin, we developed a simple one-step chromatographic procedure for thrombin purification. As a control, insoluble polystyrenes substituted only with sulphonate groups were tested. The results obtained confirmed the importance of the arginyl residues grafted onto these polymers to obtain an affinity matrix useful for purifying thrombin with a high specific activity and a good recovery.
已合成了用磺酸盐和L-精氨酰甲酯取代的不溶性聚苯乙烯。利用它们对凝血酶的特异性亲和力，我们开发了一种简单的一步色谱法来纯化凝血酶。作为对照，测试了仅用磺酸根基团取代的不溶性聚苯乙烯。所得结果证实了接枝到这些聚合物上的精氨酰残基对于获得可用于以高比活性和良好回收率纯化凝血酶的亲和基质的重要性。
