Yu X J, Fischer A M, Muller D, Bros A, Tapon-Bretaudiere J, Jozefonvicz J
J Chromatogr. 1986 Apr 11;376:429-35. doi: 10.1016/s0378-4347(00)80860-9.
Insoluble polystyrenes substituted with sulphonate and L-arginyl methyl ester (PAOM) present substituents mimicking the reactive binding site of antithrombin III. These materials have a specific affinity for thrombin. The binding of the enzyme is reversible and the eluted thrombin remains active. Consequently, these resins can be used as stationary phases in affinity liquid chromatography in order to purify thrombin with a high biological activity. The influence of different characteristics of such polymers (substitution ratio, average particle size, affinity constant, synthesis conditions) on the purification performance is studied. Human prothrombin complex concentrate is activated and applied onto the gel. A purified human thrombin of high specific activity is separated with a high recovery of biological activity of the enzyme.
用磺酸盐和L - 精氨酰甲酯（PAOM）取代的不溶性聚苯乙烯呈现出模拟抗凝血酶III反应性结合位点的取代基。这些材料对凝血酶具有特异性亲和力。酶的结合是可逆的，洗脱后的凝血酶仍保持活性。因此，这些树脂可作为亲和液相色谱中的固定相，以纯化具有高生物活性的凝血酶。研究了此类聚合物的不同特性（取代率、平均粒径、亲和常数、合成条件）对纯化性能的影响。将人凝血酶原复合物浓缩物活化后应用于凝胶上。分离得到具有高比活性的纯化人凝血酶，且酶的生物活性回收率高。
