Esteban Tomás, Atucha Noemí M, Moraleda José María, García-Estañ Joaquín, Insausti Carmen L, Moraleda-Deleyto Javier
Servicio de Otorinolaringología, Hospital General Universitario Santa Lucía, Cartagena, Spain.
Departamento de Fisiología, Facultad de Medicina, Universidad de Murcia, Instituto Murciano de Investigación Biosanitaria, 30120, El Palmar, Murcia, Spain.
J Otol. 2019 Mar;14(1):12-16. doi: 10.1016/j.joto.2018.11.002. Epub 2018 Nov 10.
A rat model of chronic tympanic membrane perforation was developed to be used in the search of new materials for the sealing of these perforations. A longitudinal study was carried out in rats subjected to incisional myringotomy followed by the application of mitomycin C alone or with dexamethasone. Rats were checked at days 3, 7, 10, 14 and weekly thereafter until perforation closure, for up to 6 months. The addition of dexamethasone is a key component in order to obtain a chronic opening. Myringotomies treated with saline had a mean healing time of 8.5 days. At 8 weeks, between 62.5% and 77.7% of tympanic membranes treated with mitomycin C and dexamethasone remained perforated and at 6 months this number fell to 21.4%. This technique is able to maintain most tympanic membrane perforations patent for at least 8 weeks. This rat model is adequate for its use in preclinical or translational research.
建立了慢性鼓膜穿孔大鼠模型，用于寻找封闭这些穿孔的新材料。对接受切开鼓膜造孔术并单独应用丝裂霉素C或联合地塞米松的大鼠进行了纵向研究。在第3、7、10、14天检查大鼠，此后每周检查一次，直至穿孔闭合，最长持续6个月。添加地塞米松是形成慢性穿孔的关键因素。用生理盐水处理的鼓膜造孔术平均愈合时间为8.5天。在8周时，用丝裂霉素C和地塞米松处理的鼓膜中，62.5%至77.7%仍有穿孔，在6个月时，这一比例降至21.4%。该技术能够使大多数鼓膜穿孔保持开放至少8周。该大鼠模型适用于临床前或转化研究。
