Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China; School of Materials Science and Engineering, University of Science and Technology of China, Shenyang, 110016, China.
Institute of Life Sciences, Wenzhou University, Wenzhou 325000, China; Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences, School of Nursing at the Wenzhou Medical University, Wenzhou 325000, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:396-410. doi: 10.1016/j.msec.2019.03.021. Epub 2019 Mar 8.
Stainless steel (SS) has been widely applied as one of the most efficient implant metal materials, although corrosion and infection in body environment are still challenging. Herein, an antibacterial passivation method was employed to enhance the antibacterial performance and corrosion resistance of the medical 316L SS. The result proved that the antibacterial-passivated 316L SS exhibited stable antibacterial activity and effectively inhibited the formation of bacterial biofilm. Electrochemical measurements combined with X-ray photoelectron spectroscopy technique were used to study the corrosion resistance and semiconductor behavior of passivated 316L SS immersed in simulated physiological environment. The results indicated that the 316L SS after antibacterial passivation treatment for 1 h, soaking in the medium for 10 days, showed satisfactory corrosion resistance attributing to proper Cu deposition in the passive film. The anodic stripping voltammetry measurement further confirmed that the Cu-bearing passive film could continuously release Cu ions into medium. The zebrafish test demonstrated an excellent in vivo biocompatibility for the 316L SS with antibacterial passivation for 0.5 and 1 h, respectively. In addition, changes of surface roughness, contact angle and chemical composition after antibacterial passivation played an important role in explaining the antibacterial mechanism, which could be clearly divided into contact killing and ionic release killing. Hence, the antibacterial passivation treatment was preliminarily proved as a potential way for enhancing the persistent antibacterial activity and corrosion resistance of 316L SS.
不锈钢（SS）已被广泛应用于最有效的植入金属材料之一，尽管在体环境中仍存在腐蚀和感染的挑战。在此，采用了一种抗菌钝化方法来提高医用 316L SS 的抗菌性能和耐腐蚀性。结果证明，抗菌钝化的 316L SS 表现出稳定的抗菌活性，并有效抑制了细菌生物膜的形成。电化学测量结合 X 射线光电子能谱技术用于研究模拟生理环境中钝化 316L SS 的耐腐蚀性和半导体行为。结果表明，经 1 h 抗菌钝化处理后的 316L SS 在介质中浸泡 10 天后表现出令人满意的耐腐蚀性，这归因于钝化膜中适当的 Cu 沉积。阳极剥离伏安法测量进一步证实，含 Cu 的钝化膜能够将 Cu 离子持续释放到介质中。斑马鱼试验证明，经过 0.5 和 1 h 抗菌钝化处理的 316L SS 具有良好的体内生物相容性。此外，抗菌钝化后的表面粗糙度、接触角和化学成分的变化在解释抗菌机制方面起着重要作用，可将其分为接触杀菌和离子释放杀菌。因此，抗菌钝化处理初步被证明是提高 316L SS 持久抗菌活性和耐腐蚀性的一种潜在方法。
