Jeleniewicz Witold, Cybulski Marek, Nowakowski Andrzej, Stenzel-Bembenek Agnieszka, Guz Małgorzata, Marzec-Kotarska Barbara, Kotarski Jan, Stepulak Andrzej
Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, Poland.
Second Department of Oncologic Gynaecology, Center of Oncology of the Lublin Region, Lublin, Poland.
Anticancer Res. 2019 Apr;39(4):1821-1827. doi: 10.21873/anticanres.13289.
BACKGROUND/AIM: Ovarian cancer is the most frequent cause of death in women among gynecological cancers in Poland. MMP-2 and MMP-9 are frequently dysregulated in cancers and they are considered as potential biomarkers. Our goal was to assess the associations between MMP-2 and MMP-9 mRNA expression, clinicopathological parameters and patients' response to chemotherapy.
We evaluated MMP-2 and MMP-9 mRNA expression in epithelial ovarian cancer (EOC) tissues from 44 untreated patients, four ovarian cancer cell lines, and human skin fibroblasts (HSF). The expression of both MMPs was estimated using qPCR.
MMP-2 expression was significantly higher (p=0.020) in EOCs sensitive to chemotherapy compared to resistant and refractory tumors. The highest MMP-2 expression was found in HSF and MMP-9 expression was the highest in EOCs (p<0.001). The expression of neither MMP was significantly associated with patients' overall survival (OS).
MMP-2 may be engaged in early stages of ovarian carcinogenesis. MMP-2 expression in EOCs may discriminate patients with a favorable response to first line chemotherapy.
背景/目的：在波兰，卵巢癌是妇科癌症中女性最常见的死亡原因。基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）在癌症中经常失调，它们被视为潜在的生物标志物。我们的目标是评估MMP-2和MMP-9 mRNA表达、临床病理参数与患者化疗反应之间的关联。
我们评估了44例未经治疗的患者的上皮性卵巢癌（EOC）组织、四种卵巢癌细胞系和人皮肤成纤维细胞（HSF）中MMP-2和MMP-9 mRNA的表达。使用定量聚合酶链反应（qPCR）估计两种基质金属蛋白酶的表达。
与耐药和难治性肿瘤相比，对化疗敏感的EOC中MMP-2表达显著更高（p = 0.020）。在HSF中发现MMP-2表达最高，而在EOC中MMP-9表达最高（p < 0.001）。两种基质金属蛋白酶的表达均与患者的总生存期（OS）无显著关联。
MMP-2可能参与卵巢癌发生的早期阶段。EOC中MMP-2的表达可能区分对一线化疗反应良好的患者。
