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一种研究抗生素抗性质粒的超快速方法。

An ultra-rapid method for the study of antibiotic resistance plasmids.

作者信息

Bennett P M, Heritage J, Hawkey P M

出版信息

J Antimicrob Chemother. 1986 Sep;18(3):421-4. doi: 10.1093/jac/18.3.421.

DOI:10.1093/jac/18.3.421
PMID:3095302
Abstract

A rapid method of isolating plasmid DNA has been developed from that of Birnboim & Doly (1979). This method allows large numbers of strains to be examined, and can be employed to isolate DNA from members of the Enterobacteriaceae, Pseudomonas aeruginosa, Staphylococcus aureus, (including methicillin-resistant strains) and coagulase-negative staphylococci. Plasmids of widely differing sizes are amenable to isolation by this technique, which yields DNA of sufficient purity to allow restriction endonuclease and homoduplex analysis.

摘要

一种快速分离质粒DNA的方法是在Birnboim和Doly(1979年)的方法基础上发展而来的。该方法能够检测大量菌株,可用于从肠杆菌科成员、铜绿假单胞菌、金黄色葡萄球菌(包括耐甲氧西林菌株)和凝固酶阴性葡萄球菌中分离DNA。各种大小差异较大的质粒都适合用这种技术进行分离,所获得的DNA纯度足以进行限制性内切酶分析和同源双链分析。

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