Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Department of Animal Sciences, Aquaculture and Fisheries Group, Wageningen University, 6708 WD, Wageningen, The Netherlands.
Appl Microbiol Biotechnol. 2019 May;103(10):4241-4252. doi: 10.1007/s00253-019-09773-4. Epub 2019 Apr 5.
Biofloc technology is an efficient approach for intensive shrimp culture. However, the extent to which this process can influence the composition of intestinal microbial community is still unknown. Here, we surveyed the shrimp intestinal bacteria as well as the floc water from three biofloc systems with different stock densities. Our study revealed a similar variation trend in phylum taxonomy level between floc bacteria and gut microbiota. Microbial community varied notably in floc water from different stock densities, while a core genus with dominating relative abundance was detected in gut samples. Extensive variation was discovered in gut microbiota, but still clustered into groups according to stock density. Our results indicated that shrimp intestinal microbiota as well as bacteria aggregated in flocs assembled into distinct communities from different stock densities, and the intestinal communities were more similar with the surrounding environment as the increase of stock density and resulting high floc biomass. The high stock density changed the core gut microbiota by reducing the relative abundance of Paracoccus and increasing that of Nocardioides, which may negatively influence shrimp performance. Therefore, this study helps us to understand further bacteria and host interactions in biofloc system.
生物絮团技术是一种集约化养殖虾类的有效方法。然而，目前尚不清楚该过程会在何种程度上影响虾肠道微生物群落的组成。在这里，我们调查了三种不同养殖密度的生物絮团系统中的虾肠道细菌和絮团水体中的细菌。研究结果表明，絮团水体中的细菌和肠道微生物在门分类水平上呈现出相似的变化趋势。不同养殖密度的絮团水体中的微生物群落存在显著差异，而在肠道样本中则检测到了一个相对丰度较高的核心属。肠道微生物群落存在广泛的变化，但仍根据养殖密度聚类。我们的研究结果表明，虾肠道微生物群落以及聚集在絮团中的细菌会根据养殖密度的不同而形成不同的群落，并且随着养殖密度的增加和絮团生物量的增加，肠道群落与周围环境更加相似。高密度养殖通过降低副球菌的相对丰度和增加诺卡氏菌的相对丰度来改变核心肠道微生物群，这可能会对虾的生长性能产生负面影响。因此，本研究有助于我们进一步了解生物絮团系统中细菌和宿主的相互作用。
