Wang Xue-Qiang, Tu Wen-Zhan, Guo Jia-Bao, Song Ge, Zhang Juan, Chen Chang-Cheng, Chen Pei-Jie
Department of Sport Rehabilitation, Shanghai University of Sport, Shanghai, China; †Department of Rehabilitation Medicine, Shanghai Shangti Orthopaedic Hospital, Shanghai, China; ‡Department of Rehabilitation Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Equal contribution.
Pain Med. 2019 Dec 1;20(12):2459-2471. doi: 10.1093/pm/pnz015.
Objectives The aim of our study was to ascertain the underlying role of microRNAs (miRNAs) in human intervertebral disc degeneration (IDD). Design Bioinformatic analysis from multiple databases. Methods Studies of the association of miRNAs and IDD were identified in multiple electronic databases. All potential studies were assessed by the same inclusion and exclusion criteria. We recorded whether miRNA expression was commonly increased or suppressed in the intervertebral disc tissues and cells of IDD subjects. We used String to identify biological process and cellular component pathways of differentially expressed genes. Results We included fifty-seven articles from 1,277 records in this study. This report identified 40 different dysregulated miRNAs in 53 studies, including studies examining cell apoptosis (26 studies, 49.06%), cell proliferation (15 studies, 28.3%), extracellular matrix (ECM) degradation (10 studies, 18.86%), and inflammation (five studies, 9.43%) in IDD patients. Three upregulated miRNAs (miR-19b, miR-32, miR-130b) and three downregulated miRNAs (miR-31, miR-124a, miR-127-5p) were considered common miRNAs in IDD tissues. The top three biological process pathways for upregulated miRNAs were positive regulation of biological process, nervous system development, and negative regulation of biological process, and the top three biological process pathways for downregulated miRNAs were negative regulation of gene expression, intracellular signal transduction, and negative regulation of biological process. Conclusions This study revealed that miRNAs could be novel targets for preventing IDD and treating patients with IDD by regulating their target genes. These results provide valuable information for medical professionals, IDD patients, and health care policy makers.
目的 我们研究的目的是确定微小RNA（miRNA）在人类椎间盘退变（IDD）中的潜在作用。
设计 来自多个数据库的生物信息学分析。
方法 在多个电子数据库中鉴定miRNA与IDD关联的研究。所有潜在研究均根据相同的纳入和排除标准进行评估。我们记录了IDD受试者椎间盘组织和细胞中miRNA表达是普遍增加还是受到抑制。我们使用String来识别差异表达基因的生物学过程和细胞成分途径。
结果 本研究纳入了1277条记录中的57篇文章。该报告在53项研究中鉴定出40种不同的失调miRNA，包括研究IDD患者细胞凋亡（26项研究，49.06%）、细胞增殖（15项研究，28.3%）、细胞外基质（ECM）降解（10项研究，18.86%）和炎症（5项研究，9.43%）的研究。三种上调的miRNA（miR-19b、miR-32、miR-130b）和三种下调的miRNA（miR-31、miR-124a、miR-127-5p）被认为是IDD组织中常见的miRNA。上调miRNA的前三大生物学过程途径是生物过程的正调控、神经系统发育和生物过程的负调控，下调miRNA的前三大生物学过程途径是基因表达的负调控、细胞内信号转导和生物过程的负调控。
结论 本研究表明，miRNA可能是通过调节其靶基因来预防IDD和治疗IDD患者的新靶点。这些结果为医学专业人员、IDD患者和医疗保健政策制定者提供了有价值的信息。
