Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, 530005, People's Republic of China.
Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530025, People's Republic of China.
Biol Res. 2019 Apr 6;52(1):20. doi: 10.1186/s40659-019-0227-6.
Histone acetylation is an important epigenetic modification that regulates gene activity in response to stress. Histone acetylation levels are reversibly regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). The imperative roles of HDACs in gene transcription, transcriptional regulation, growth and responses to stressful environment have been widely investigated in Arabidopsis. However, data regarding HDACs in kenaf crop has not been disclosed yet.
In this study, six HDACs genes (HcHDA2, HcHDA6, HcHDA8, HcHDA9, HcHDA19, and HcSRT2) were isolated and characterized. Phylogenetic tree revealed that these HcHDACs shared high degree of sequence homology with those of Gossypium arboreum. Subcellular localization analysis showed that GFP-tagged HcHDA2 and HcHDA8 were predominantly localized in the nucleus, HcHDA6 and HcHDA19 in nucleus and cytosol. The HcHDA9 was found in both nucleus and plasma membranes. Real-time quantitative PCR showed that the six HcHDACs genes were expressed with distinct expression patterns across plant tissues. Furthermore, we determined differential accumulation of HcHDACs transcripts under salt and drought treatments, indicating that these enzymes may participate in the biological process under stress in kenaf. Finally, we showed that the levels of histone H3 and H4 acetylation were modulated by salt and drought stress in kenaf.
We have isolated and characterized six HDACs genes from kenaf. These data showed that HDACs are imperative players for growth and development as well abiotic stress responses in kenaf.
组蛋白乙酰化是一种重要的表观遗传修饰，可调节应激条件下的基因活性。组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）可使组蛋白乙酰化水平可逆调节。HDACs 在基因转录、转录调控、生长以及对应激环境的响应中的重要作用已在拟南芥中得到广泛研究。然而，有关麻疯树作物中 HDACs 的数据尚未披露。
本研究分离并鉴定了六个 HDACs 基因（HcHDA2、HcHDA6、HcHDA8、HcHDA9、HcHDA19 和 HcSRT2）。系统进化树分析表明，这些 HcHDACs 与棉属的 Gossypium arboreum 具有高度的序列同源性。亚细胞定位分析表明，GFP 标记的 HcHDA2 和 HcHDA8 主要定位于细胞核，HcHDA6 和 HcHDA19 定位于细胞核和细胞质。HcHDA9 则存在于细胞核和质膜中。实时定量 PCR 显示，六个 HcHDACs 基因在不同组织中的表达模式存在明显差异。此外，我们还确定了 HcHDACs 转录本在盐和干旱处理下的差异积累，表明这些酶可能参与麻疯树的生物过程。最后，我们表明盐和干旱胁迫可调节麻疯树中的组蛋白 H3 和 H4 乙酰化水平。
我们从麻疯树中分离并鉴定了六个 HDACs 基因。这些数据表明，HDACs 是麻疯树生长发育以及非生物胁迫响应的重要参与者。
