Mao Lei, Wang Fei, Li Yuanyuan, Dai Yufeng, Liu Yanjun, Wang Jingfeng, Xue Changhu
College of Food Science and Engineering, Ocean University of China, Qingdao, 266003 Shandong Province China.
Food Sci Biotechnol. 2018 Sep 14;28(2):539-545. doi: 10.1007/s10068-018-0463-5. eCollection 2019 Apr.
Glucocorticoids are the leading cause of secondary osteoporosis. In the current study, the in vivo effects of Antarctic krill () oil (AKO) on dexamethasone-treated mice were investigated. Results showed that AKO significantly prevents bone loss, as evidenced by improved bone mineral density, biomechanical strength, and cancellous bone microstructure. Fluorescence double-labeling of femur showed that AKO induces new bone formation. Toluidine blue staining of marrow cavity indicated that AKO increases the number of trabecula, and decreases the generation of adipose cells. Runt-related transcription factor 2 (Runx2) and Peroxisome proliferator-activated receptor γ (PPARγ) are the switches for osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells, respectively. AKO significantly promoted the expression of Runx2 protein, and reduced PPARγ expression in bone tissue. Furthermore, AKO increased the mRNA expression of osteogenesis-related genes and decreased the expression of adipogenesis-related genes. In conclusion, AKO improved glucocorticoid-induced osteoporosis via promoting bone formation.
糖皮质激素是继发性骨质疏松症的主要病因。在本研究中，研究了南极磷虾油（AKO）对用 dexamethasone 处理的小鼠的体内作用。结果表明，AKO 显著预防骨质流失，这通过改善骨矿物质密度、生物力学强度和松质骨微观结构得以证明。股骨的荧光双标记显示 AKO 诱导新骨形成。骨髓腔的甲苯胺蓝染色表明 AKO 增加小梁数量，并减少脂肪细胞的生成。 runt 相关转录因子 2（Runx2）和过氧化物酶体增殖物激活受体γ（PPARγ）分别是骨髓间充质干细胞成骨和成脂分化的开关。AKO 显著促进 Runx2 蛋白的表达，并降低骨组织中 PPARγ 的表达。此外，AKO 增加成骨相关基因的 mRNA 表达，并降低成脂相关基因的表达。总之，AKO 通过促进骨形成改善糖皮质激素诱导的骨质疏松症。
