Almeida-da-Silva Cássio Luiz Coutinho, Alpagot Tamer, Zhu Ye, Lee Sonho Sierra, Roberts Brian P, Hung Shu-Chen, Tang Norina, Ojcius David M
Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, CA 94103, USA.
Department of Periodontics, Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, CA 94103, USA.
Microb Cell. 2019 Mar 11;6(4):197-208. doi: 10.15698/mic2019.04.674.
is an airborne, Gram-negative, obligate intracellular bacterium which causes human respiratory infections and has been associated with atherosclerosis. Because individuals with periodontitis are at greater risk for atherosclerosis as well as respiratory infections, we in-vestigated the role of in inflammation and periodontal dis-ease. We found that was more frequently found in subgingival dental plaque obtained from periodontally diseased sites of the mouth versus healthy sites. The known periodontal pathogens, and , were also found in the plaque. In addition, could efficiently invade human gingival epithelial cells (GECs) , causing translocation of NF-κB to the nucleus along with increased secretion of mature IL-1β cytokine. Supernatants collected from -infected GECs showed increased activation of caspase-1 protein, which was significantly reduced when gene expression was silenced using shRNA lentiviral vectors. Our results demonstrate that was found in higher levels in periodontitis patients compared to control pa-tients. Additionally, could infect GECs, leading to inflammation caused by activation of NF-κB and the NLRP3 inflammasome. We propose that the presence of in subgingival dental plaque may contribute to periodontal disease and could be used as a potential risk indicator of perio-dontal disease.
是一种空气传播的革兰氏阴性专性细胞内细菌，可引起人类呼吸道感染，并与动脉粥样硬化有关。由于牙周炎患者患动脉粥样硬化和呼吸道感染的风险更高，我们研究了其在炎症和牙周疾病中的作用。我们发现，与健康部位相比，在从口腔牙周病部位获取的龈下牙菌斑中更频繁地发现该细菌。在菌斑中还发现了已知的牙周病原体和。此外，该细菌能够有效侵入人牙龈上皮细胞（GECs），导致NF-κB易位至细胞核，同时成熟IL-1β细胞因子的分泌增加。从感染该细菌的GECs收集的上清液显示caspase-1蛋白的激活增加，当使用shRNA慢病毒载体沉默该细菌基因表达时，激活显著降低。我们的结果表明，与对照患者相比，牙周炎患者中该细菌的水平更高。此外，该细菌可感染GECs，导致由NF-κB和NLRP3炎性小体激活引起的炎症。我们认为龈下牙菌斑中该细菌的存在可能导致牙周疾病，并可作为牙周疾病的潜在风险指标。
