Rios Manolo U, Bucksot Jesse E, Rahebi Kimiya C, Engineer Crystal T, Kilgard Michael P, Hays Seth A
School of Brain and Behavioral Sciences, The University of Texas at Dallas, Richardson, TX 75080-3021, USA;
School of Biomedical Engineering, The University of Texas at Dallas, Richardson, TX 75080-3021, USA;
Methods Protoc. 2019 Mar;2(1). doi: 10.3390/mps2010019. Epub 2019 Feb 15.
Peripheral nerve stimulation has emerged as a platform therapy to treat a wide range of disorders. Continued development and translation of these strategies requires that researchers have access to reliable, customizable electrodes for nerve stimulation. Here, we detail procedures to build three different configurations of cuff electrodes with varying numbers and orientations of contacts for nerve stimulation in rats. These designs are built with simple, widely available materials, using platinum-iridium electrodes assembled into polyurethane tubing. Moreover, the designs can easily be customized to increase versatility and individualize for specific stimulation applications. This protocol provides a resource to facilitate the construction and customization of stimulation cuffs to support preclinical nerve stimulation research.
外周神经刺激已成为一种治疗多种疾病的平台疗法。这些策略的持续发展和转化要求研究人员能够获得用于神经刺激的可靠、可定制电极。在此，我们详细介绍构建三种不同配置袖带电极的程序，这些电极具有不同数量和方向的触点，用于大鼠的神经刺激。这些设计采用简单、广泛可用的材料制成，使用组装在聚氨酯管中的铂铱电极。此外，这些设计可以轻松定制，以增加通用性并针对特定刺激应用进行个性化设计。本方案提供了一种资源，以促进刺激袖带的构建和定制，以支持临床前神经刺激研究。
