Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, 76131 Karlsruhe, Germany.
Laboratory of Molecular Biology, Nutrition and Biotechnology, Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain.
Nutrients. 2019 Apr 6;11(4):796. doi: 10.3390/nu11040796.
() comprise several lipophilic constituents with proposed anti-obesity and anti-diabetic properties. We investigated the effect of an ethanolic extract (PTE) on energy metabolism in obesity-prone mice fed a high fat diet (HFD). Six- to eight-week-old male C57BL/6J mice were switched to HFD and, at the same time, received orally placebo or PTE (100 mg or 300 mg/kg body weight/day). Body weight, body composition, and food intake were monitored. After 26 days, blood and tissue samples were collected for biochemical, morphological, and gene expression analyses. PTE-supplemented mice accumulated fucoxanthin metabolites in adipose tissues and attained lower body weight gain, body fat content, weight of white adipose tissue (WAT) depots, and inguinal WAT adipocyte size than controls, independent of decreased food intake. PTE supplementation was associated with lower expression of (a marker of fat tissue expandability) in WAT depots, lower gene expression related to lipid uptake and turnover in visceral WAT, increased expression of genes key to fatty acid oxidation and thermogenesis (, ) in subcutaneous WAT, and signs of thermogenic activation including enhanced UCP1 protein in interscapular brown adipose tissue. In conclusion, these data show the potential of PTE to ameliorate HFD-induced obesity in vivo.
（）包含几种具有抗肥胖和抗糖尿病特性的亲脂性成分。我们研究了一种乙醇提取物（PTE）对高脂肪饮食（HFD）喂养的肥胖易感小鼠能量代谢的影响。6 至 8 周龄雄性 C57BL/6J 小鼠切换到 HFD，并同时口服安慰剂或 PTE（100 mg 或 300 mg/kg 体重/天）。监测体重、身体成分和食物摄入量。26 天后，采集血液和组织样本进行生化、形态和基因表达分析。补充 PTE 的小鼠在脂肪组织中积累了岩藻黄质代谢物，与对照组相比，体重增加、体脂肪含量、白色脂肪组织（WAT）储存的重量和腹股沟 WAT 脂肪细胞大小降低，而食物摄入量减少。PTE 补充与 WAT 储存中（脂肪组织扩张的标志物）的表达降低、内脏 WAT 中与脂质摄取和周转相关的基因表达降低、皮下 WAT 中与脂肪酸氧化和产热相关的基因表达增加（、）以及肩胛间棕色脂肪组织中 UCP1 蛋白增强等产热激活迹象相关。总之，这些数据表明 PTE 具有改善体内 HFD 诱导肥胖的潜力。
