牛奶中单核细胞增生李斯特菌的流式细胞术检测方法。
Method for flow cytometric detection of Listeria monocytogenes in milk.
作者信息
Donnelly C W, Baigent G J
出版信息
Appl Environ Microbiol. 1986 Oct;52(4):689-95. doi: 10.1128/aem.52.4.689-695.1986.
Abstract
This report describes a method for the detection of Listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. The use of immunofluorescence in combination with measures of DNA content by propidium iodide labeling and size by light scattering enabled specific identification of L. monocytogenes from Streptococcus faecalis, Streptococcus agalactiae, Streptococcus uberis, Staphylococcus epidermidis, and Staphylococcus hyicus. Additional specific resolution of L. monocytogenes populations was achieved through selective enrichment of raw milk in Listeria enrichment broth. These procedures should permit the rapid screening of milk and other food samples for L. monocytogenes and eliminate many of the short-comings associated with conventional fluorescent-antibody procedures.
摘要
本报告描述了一种通过对荧光标记细菌群体进行流式细胞术分析来检测生牛奶中单核细胞增生李斯特菌的方法。将免疫荧光与碘化丙啶标记的DNA含量测量及光散射测量的大小相结合，能够从粪肠球菌、无乳链球菌、乳房链球菌、表皮葡萄球菌和猪葡萄球菌中特异性鉴定单核细胞增生李斯特菌。通过在李斯特菌增菌肉汤中对生牛奶进行选择性增菌，实现了对单核细胞增生李斯特菌群体的进一步特异性分辨。这些程序应能快速筛查牛奶和其他食品样本中的单核细胞增生李斯特菌，并消除许多与传统荧光抗体程序相关的缺点。
相似文献
1
Method for flow cytometric detection of Listeria monocytogenes in milk.牛奶中单核细胞增生李斯特菌的流式细胞术检测方法。
Appl Environ Microbiol. 1986 Oct;52(4):689-95. doi: 10.1128/aem.52.4.689-695.1986.
2
Flow cytometry for automated analysis of milk containing Listeria monocytogenes.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):655-8.
3
Detection of Listeria monocytogenes in milk by the polymerase chain reaction.用聚合酶链反应检测牛奶中的单核细胞增生李斯特菌。
Zentralbl Bakteriol. 1999 Feb;289(1):31-6. doi: 10.1016/s0934-8840(99)80120-0.
4
Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction.利用聚合酶链反应灵敏且特异检测牛奶和碎牛肉中的单核细胞增生李斯特菌。
Appl Environ Microbiol. 1991 Sep;57(9):2576-80. doi: 10.1128/aem.57.9.2576-2580.1991.
5
Occurrence of Listeria species in raw milk and dairy products produced in Northern Ireland.北爱尔兰生产的生牛奶和乳制品中李斯特菌属的出现情况。
J Appl Bacteriol. 1992 Feb;72(2):119-25. doi: 10.1111/j.1365-2672.1992.tb01812.x.
6
7
Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments.通过体外扩增溶血素基因片段检测和鉴定熟香肠制品及牛奶中的单核细胞增生李斯特菌。
J Appl Bacteriol. 1991 May;70(5):372-9. doi: 10.1111/j.1365-2672.1991.tb02951.x.
8
[Use of PCR methods for identification of Listeria monocytogenes in milk].[使用聚合酶链反应（PCR）方法鉴定牛奶中的单核细胞增生李斯特菌]
Med Dosw Mikrobiol. 1998;50(3-4):251-7.
9
Development of a random genomic DNA microarray for the detection and identification of Listeria monocytogenes in milk.开发一种随机基因组 DNA 微阵列，用于检测和鉴定牛奶中的单核细胞增生李斯特菌。
Int J Food Microbiol. 2013 Feb 1;161(2):134-41. doi: 10.1016/j.ijfoodmicro.2012.11.023. Epub 2012 Dec 6.
10
Rapid detection of Listeria monocytogenes in food by polymerase chain reaction.通过聚合酶链反应快速检测食品中的单核细胞增生李斯特菌。
Cell Mol Biol (Noisy-le-grand). 2009 Feb 25;55 Suppl:OL1104-10.
引用本文的文献
1
16 Methods for Detecting Microbial Pathogens in Food and Water.16种食品和水中微生物病原体的检测方法。
Methods Microbiol. 1990;22:497-531. doi: 10.1016/S0580-9517(08)70253-8. Epub 2008 Apr 28.
2
Prevalence, virulence genes, and antimicrobial resistance profile of isolated from retail poultry shops in Beni-Suef city, Egypt.从埃及贝尼苏韦夫市零售家禽店分离出的[具体内容未给出]的流行率、毒力基因和抗菌药物耐药性概况。
J Adv Vet Anim Res. 2020 Dec 3;7(4):710-717. doi: 10.5455/javar.2020.g472. eCollection 2020 Dec.
3
Protein A-Mediated Binding of spp. to Antibodies in Flow Cytometric Assays and Reduction of This Binding by Using Fc Receptor Blocking Reagent.蛋白 A 介导的 spp. 与流式细胞术分析中抗体的结合，以及使用 Fc 受体阻断剂减少这种结合。
Appl Environ Microbiol. 2020 Aug 18;86(17). doi: 10.1128/AEM.01435-20.
4
An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food.食品中单核细胞增生李斯特菌的分离、计数及分子检测研究
Front Microbiol. 2015 Nov 3;6:1227. doi: 10.3389/fmicb.2015.01227. eCollection 2015.
5
Listeriosis in human pregnancy: a systematic review.人类妊娠李斯特菌病：系统评价。
J Perinat Med. 2011 May;39(3):227-36. doi: 10.1515/jpm.2011.035. Epub 2011 Apr 25.
6
Tracking bacterial infection of macrophages using a novel red-emission pH sensor.利用新型红色发射 pH 传感器跟踪巨噬细胞中的细菌感染。
Anal Bioanal Chem. 2010 Oct;398(3):1375-84. doi: 10.1007/s00216-010-4060-6. Epub 2010 Aug 19.
7
Flow cytometry applications in the food industry.流式细胞术在食品工业中的应用。
J Ind Microbiol Biotechnol. 2009 Aug;36(8):999-1011. doi: 10.1007/s10295-009-0608-x. Epub 2009 Jun 26.
8
A flow-cytometric gram-staining technique for milk-associated bacteria.一种用于牛奶相关细菌的流式细胞术革兰氏染色技术。
Appl Environ Microbiol. 2003 May;69(5):2857-63. doi: 10.1128/AEM.69.5.2857-2863.2003.
9
Detection of bacterial contamination in starch and resin-based papermaking chemicals using fluorescence techniques.
J Ind Microbiol Biotechnol. 2003 Apr;30(4):239-44. doi: 10.1007/s10295-003-0046-0. Epub 2003 Apr 26.
10
Safety and shedding of an attenuated strain of Listeria monocytogenes with a deletion of actA/plcB in adult volunteers: a dose escalation study of oral inoculation.成人志愿者中缺失actA/plcB的减毒单核细胞增生李斯特菌菌株的安全性及脱落情况：口服接种的剂量递增研究
Infect Immun. 2002 Jul;70(7):3592-601. doi: 10.1128/IAI.70.7.3592-3601.2002.
本文引用的文献
1
Role of Protein A in Nonspecific Immunofluorescence of Staphylococcus aureus.金黄色葡萄球菌非特异性免疫荧光中蛋白 A 的作用。
Infect Immun. 1970 Oct;2(4):387-91. doi: 10.1128/iai.2.4.387-391.1970.
2
The use of potassium tellurite, sodium azide, and acetic acid in a selective medium for the isolation of Listeria monocytogenes.在用于分离单核细胞增生李斯特菌的选择性培养基中使用亚碲酸钾、叠氮化钠和乙酸。
J Bacteriol. 1950 Mar;59(3):443-4. doi: 10.1128/jb.59.3.443-444.1950.
3
Is the central dogma of flow cytometry true: that fluorescence intensity is proportional to cellular dye content?
Cytometry. 1982 Sep;3(2):71-8. doi: 10.1002/cyto.990030202.
4
New methodology for the isolation of Listeria microorganisms from heavily contaminated environments.从严重污染环境中分离李斯特菌微生物的新方法。
Appl Environ Microbiol. 1984 May;47(5):1188-90. doi: 10.1128/aem.47.5.1188-1190.1984.
5
Epidemic listeriosis--evidence for transmission by food.流行性李斯特菌病——通过食物传播的证据。
N Engl J Med. 1983 Jan 27;308(4):203-6. doi: 10.1056/NEJM198301273080407.
6
Automated immunofluorescent speciation of oral bacteria using flow cytometry.使用流式细胞术对口腔细菌进行自动免疫荧光分类鉴定
J Dent Res. 1984 Aug;63(8):1040-2. doi: 10.1177/00220345840630080501.
7
Bacterial characterization by flow cytometry.通过流式细胞术进行细菌鉴定
Science. 1983 May 6;220(4597):620-2. doi: 10.1126/science.6188215.
8
Listeria monocytogenes and listeric infections.单核细胞增生李斯特菌与李斯特菌感染
Bacteriol Rev. 1966 Jun;30(2):309-82. doi: 10.1128/br.30.2.309-382.1966.
9
[Medium with nalidixic acid for isolation of streptococci, D. pneumoniae, Listeria and Erysipelothrix].含萘啶酸的培养基用于分离链球菌、肺炎链球菌、李斯特菌和红斑丹毒丝菌
Ann Inst Pasteur (Paris). 1966 Jul;111(1):90-3.
10
Differential media in the isolation of Listeria monocytogenes.
Zentralbl Bakteriol Orig A. 1973 Aug;224(3):362-75.
Zotero 插件