Harun Al Rashid Md, Majumder Sayani, Mandal Vivekananda, Mandal Subhash C, Thandavarayan Rajarajan Amirthalingam
Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
Institute of Pharmacy, Guru Ghasidas Central University, Bilaspur 495009, India.
J Tradit Complement Med. 2018 Apr 30;9(2):106-118. doi: 10.1016/j.jtcme.2017.11.003. eCollection 2019 Apr.
Roxb. () belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its antidiabetic prospective of the leaves of Qualitative phytochemicals analysis of the samples from was carried out for the detection of secondary metabolites. Total phenolics, flavonoids, triterpenoids and tannins content of were estimated using colorimetric assay. Microwave-assisted extraction (MAE) technique with a low carbon output was observed for the speedy extraction of bioactive compounds obtained from leaf extract. MAE produced a maximum yield of bioactive compounds which was found to be more efficient than ultrasound, soxhlet and maceration extraction. Qualitative HPLC analysis was performed for bioactive compounds. The antidiabetic assay was performed using α-amylase and α-glucosidase inhibitory activity. In conclusion, the fractions exhibited the concentration-dependent inhibitory effect with significant ( < 0.0001) result. So the above performance might be accountable for the antidiabetic activity of leaf extract due to presence of bioactive compounds.
罗克斯伯（）属于柿科，其叶子因用于制作比迪烟而在全世界广为人知。当前的研究估计了比较提取技术及其对 叶的抗糖尿病前景。对来自 的样本进行了定性植物化学分析，以检测次生代谢产物。使用比色法估计了 中的总酚、黄酮、三萜和单宁含量。观察到具有低碳输出的微波辅助提取（MAE）技术可快速提取从 叶提取物中获得的生物活性化合物。MAE产生的生物活性化合物产量最高，发现其比超声、索氏提取和浸渍提取更有效。对生物活性化合物进行了定性HPLC分析。使用α-淀粉酶和α-葡萄糖苷酶抑制活性进行了抗糖尿病测定。总之，这些馏分表现出浓度依赖性抑制作用，结果具有显著性（<0.0001）。因此，由于存在生物活性化合物，上述性能可能是 叶提取物抗糖尿病活性的原因。
