Research Centre of Excellence, Nutrition and Non-Communicable Diseases, Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Malaysian Agricultural Research and Development Institute, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia.
Research Centre of Excellence, Nutrition and Non-Communicable Diseases, Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Food Chem. 2020 Nov 30;331:127240. doi: 10.1016/j.foodchem.2020.127240. Epub 2020 Jun 17.
The present study focused on the phytochemical profiling along with evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of various crudes and fractions obtained from Lepisanthes fruticosa (Roxb) Leenh fruit. Ethanolic seed crude extract exhibited the strongest radical scavenging, β-carotene bleaching activity, α-glucosidase inhibition and the highest total phenolic content (TPC). Column chromatography afforded various fractions with fraction M4 being the most potent due to the strongest radical scavenging, β-carotene bleaching, α-glucosidase inhibition and greatest amount of TPC. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of ethanolic seed crude extract and fraction M4 showed the presence of various phytochemicals with antioxidant and antidiabetic properties, which include mostly flavonoids and tannins. The results may suggest that the ethanolic crude seed extract and its fraction could be an excellent source of bioactive phytochemicals with antioxidant and antidiabetic potential.
本研究集中于植物化学成分分析，并评估 Lepisanthes fruticosa (Roxb) Leenh 果实不同粗提物和馏分的体外抗氧化、α-葡萄糖苷酶和α-淀粉酶抑制活性。乙醇种子粗提取物表现出最强的自由基清除、β-胡萝卜素漂白活性、α-葡萄糖苷酶抑制作用和最高的总酚含量（TPC）。柱层析得到的各种馏分中，馏分 M4 的活性最强，因为它具有最强的自由基清除、β-胡萝卜素漂白、α-葡萄糖苷酶抑制作用和最高的 TPC 含量。乙醇种子粗提取物和馏分 M4 的液质联用分析（LC-MS/MS）表明，存在具有抗氧化和抗糖尿病特性的各种植物化学成分，主要包括黄酮类和单宁类。结果表明，乙醇粗种子提取物及其馏分可能是具有抗氧化和抗糖尿病潜力的生物活性植物化学成分的极好来源。
