School of Life Sciences, Jilin University, Changchun 130012, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, School of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Nutrients. 2022 Aug 12;14(16):3299. doi: 10.3390/nu14163299.
(Bull.: Fr.) P. Karst. spore powder (IHS) contains polyphenols and triterpenoids with pharmacological effects. Here, we analyzed its composition, and we investigated the effects of IHS on colorectal cancer (CRC) in B6/JGpt-/Gpt () mice and its potential mechanisms by analyzing gut microbiota and serum metabolomics. The enzyme-linked immunosorbent assays and Western blotting were used to confirm the changes in the cytokine and protein levels associated with IHS administration. The IHS affected the abundance of gut microbiota and the level of -arginine (-Arg). Furthermore, the IHS influenced T cells in mice by increasing the interleukin (IL)-2 and decreasing the IL-5, -6, and -10 levels, thus suppressing tumor development. Overall, IHS showed anti-CRC properties in mice by affecting the gut microbiota and serum metabolites, which in turn affected the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling, and regulated the abundance of CD8 T cells. These results provide experimental support for the potential future treatment of CRC with IHS.
（Bull.：Fr.）P. Karst. 孢子粉（IHS）含有具有药理作用的多酚和三萜类化合物。在这里，我们分析了它的组成，并通过分析肠道微生物群和血清代谢组学，研究了 IHS 对 B6/JGpt-/Gpt（）小鼠结直肠癌（CRC）的影响及其潜在机制。酶联免疫吸附试验和 Western blotting 用于确认与 IHS 给药相关的细胞因子和蛋白质水平的变化。IHS 影响肠道微生物群的丰度和 -精氨酸（-Arg）的水平。此外，IHS 通过增加白细胞介素（IL）-2 和降低 IL-5、-6 和 -10 的水平影响 小鼠中的 T 细胞，从而抑制肿瘤的发展。总的来说，IHS 通过影响肠道微生物群和血清代谢物，进而影响 Janus 激酶（JAK）/信号转导和转录激活因子（STAT）信号，调节 CD8 T 细胞的丰度，在 小鼠中显示出抗 CRC 特性。这些结果为未来使用 IHS 治疗 CRC 提供了实验支持。
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