Young Researcher's Society, Shahid Bahonar University of Kerman, Kerman, Iran.
Biology Department, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran.
IET Nanobiotechnol. 2019 Feb;13(1):66-70. doi: 10.1049/iet-nbt.2018.5212.
The interaction between nanoparticles and plants is inevitable. In this study, the effect of different concentrations of ZnO nanoparticles synthesised using olive extract on the soybean was studied. The soybean seeds were cultured in a Hoagland medium containing agar which was treated different concentrations (0, 200 and 400 ppm) of ZnO nanoparticles. After 21 days, the plants were harvested and the parameters of proline, anthocyanin, malondialdehyde (MDA), hydrogen peroxide (HO), chlorophyll and carotenoid contents and phenylalanine ammonia-lyase (PAL) and catalase (CAT) activity in soybeans () were measured. The results showed that the levels of chlorophyll and and carotenoid at concentrations of 200 and 400 ppm in comparison with control decreased, while carotenoid content at 200 ppm concentration at a concentration of 400 ppm was not significant. The level of anthocyanin and PAL activity increased with increasing concentration of nanoparticles, while proline content decreased. By increasing the concentration of ZnO nanoparticles, the content of MDA and hydrogen peroxide increased compared to control but CAT activity did not change significantly. This research suggests that ZnO nanoparticles synthesised using olive extract in soybean plants may be toxic by reactive oxygen species production.
纳米粒子与植物之间的相互作用是不可避免的。在这项研究中，研究了用橄榄油提取物合成的不同浓度的 ZnO 纳米粒子对大豆的影响。将大豆种子在含有琼脂的 Hoagland 培养基中培养，琼脂用不同浓度（0、200 和 400 ppm）的 ZnO 纳米粒子处理。21 天后，收获植物，并测量大豆中脯氨酸、花青素、丙二醛（MDA）、过氧化氢（HO）、叶绿素和类胡萝卜素含量以及苯丙氨酸解氨酶（PAL）和过氧化氢酶（CAT）活性的参数。结果表明，与对照相比，200 和 400 ppm 浓度下的叶绿素和含量降低，而 400 ppm 浓度下的类胡萝卜素含量无显著差异。随着纳米粒子浓度的增加，花青素和 PAL 活性增加，而脯氨酸含量降低。与对照相比，随着 ZnO 纳米粒子浓度的增加，MDA 和过氧化氢的含量增加，但 CAT 活性没有明显变化。这项研究表明，用橄榄油提取物合成的 ZnO 纳米粒子在大豆植株中可能通过产生活性氧而具有毒性。
