Soltani Nejad Meysam, Khatami Mehrdad, Shahidi Bonjar Gholam Hosein
Department of Plant Pathology, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Biotechnology, Shahid Bahonar University of Kerman, Kerman, Iran.
IET Nanobiotechnol. 2016 Feb;10(1):33-8. doi: 10.1049/iet-nbt.2015.0028.
Applications of nanotechnology and nano-science have ever-expanding breakthroughs in medicine, agriculture and industries in recent years; therefore, synthesis of metals nanoparticle (NP) has special significance. Synthesis of NPs by chemical methods are long, costly and hazardous for environment so biosynthesis has been developing interest for researchers. In this regard, the extracellular biosynthesis of gold nanotriangles (AuNTs) performed by use of the soil Streptomycetes. Streptomycetes isolated from rice fields of Guilan Province, Iran, showed biosynthetic activity for producing AuNTs via in vitro experiments. Among all 15 Streptomyces spp. isolates, isolate No. 5 showed high biosynthesis activity. To determine the bacterium taxonomical identity at genus level, its colonies characterised morphologically by use of scanning electron microscope. The polymerase chain reaction (PCR) molecular analysis of active isolate represented its identity partially. In this regard, 16S rRNA gene of the isolate was amplified using universal bacterial primers FD1 and RP2. The PCR products were purified and sequenced. Sequence analysis of 16S rDNA was then conducted using National Center for Biotechnology Information Basic Local Alignment Search Tool method. The AuNTs obtained were characterised by ultraviolet-visible spectroscopy, atomic force microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction spectroscopy analyses. The authors results indicated that Streptomyces microflavus isolate 5 bio-synthesises extracellular AuNTs in the range of 10-100 nm. Synthesised SNPs size ranged from 10 to 100 nm. In comparison with chemical methods for synthesis of metal NPs, the biosynthesis of AuNTs by Streptomyces source is a fast, simple and eco-friendly method. The isolate is a good candidate for further investigations to optimise its production efficacy for further industrial goals in biosynthesis of AuNTs.
近年来，纳米技术和纳米科学在医学、农业和工业领域取得了不断扩展的突破；因此，金属纳米颗粒（NP）的合成具有特殊意义。通过化学方法合成纳米颗粒耗时较长、成本高昂且对环境有害，所以生物合成法已引起研究人员的兴趣。在这方面，利用土壤链霉菌进行了金纳米三角形（AuNTs）的细胞外生物合成。从伊朗吉兰省稻田分离出的链霉菌通过体外实验显示出产生AuNTs的生物合成活性。在所有15株链霉菌属分离株中，5号分离株表现出较高的生物合成活性。为了在属水平确定该细菌的分类学身份，利用扫描电子显微镜对其菌落进行了形态学表征。对活性分离株的聚合酶链反应（PCR）分子分析部分显示了其身份。在这方面，使用通用细菌引物FD1和RP2扩增了该分离株的16S rRNA基因。对PCR产物进行了纯化和测序。然后使用美国国立生物技术信息中心基本局部比对搜索工具方法对16S rDNA进行了序列分析。通过紫外可见光谱、原子力显微镜、透射电子显微镜、能量色散X射线光谱、傅里叶变换红外光谱（FTIR）和X射线衍射光谱分析对获得的AuNTs进行了表征。作者的结果表明，微黄链霉菌分离株5在细胞外生物合成10 - 100nm范围内的AuNTs。合成的单核苷酸多态性（SNPs）尺寸范围为10至100nm。与化学合成金属纳米颗粒的方法相比，利用链霉菌来源生物合成AuNTs是一种快速、简单且环保的方法。该分离株是进一步研究以优化其生产效率以实现AuNTs生物合成中进一步工业目标的良好候选对象。
