Department of Urology, Tongji Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.
Department of General Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.
J Cell Biochem. 2019 Aug;120(8):13487-13493. doi: 10.1002/jcb.28622. Epub 2019 Mar 27.
Long noncoding RNAs (lncRNAs) CASC11 is an oncogenic lncRNA in gastric cancer and colorectal cancer. Our study aimed to investigate the role of lncRNA CASC11 in bladder cancer. In this study we showed that plasma lncRNA CASC11 was upregulated, while plasma miRNA-150 was downregulated in patients with early-stage bladder cancer than in healthy controls. Altered expression of plasma lncRNA CASC11 and miRNA-150 separated patients with bladder cancer from healthy controls. lncRNA CASC11 expression was inversely correlated with miRNA-150 expression in patients with bladder cance but not in healthy controls. Overexpression of lncRNA CASC11 mediated the inhibition of miRNA-150 expression in cancer cells, while miRNA-150 overexpression did not significantly alter lncRNA CASC11 expression. lncRNA CASC11 overexpression promoted, while miRNA-150 overexpression inhibited cancer cell proliferation. miRNA-150 also attenuated the enhancing effects of lncRNA CASC11 overexpression on cancer cell proliferation. However, overexpression of lncRNA CASC11 showed no significant effects on cancer cell migration and invasion. Therefore, lncRNA CASC11 may promote cancer cell proliferation in bladder cancer, and the actions of lncRNA CASC11 are likely through miRNA-150.
长链非编码 RNA (lncRNA) CASC11 是胃癌和结直肠癌的致癌 lncRNA。本研究旨在探讨 lncRNA CASC11 在膀胱癌中的作用。在这项研究中，我们发现早期膀胱癌患者的血浆 lncRNA CASC11 上调，而血浆 miRNA-150 下调。血浆 lncRNA CASC11 和 miRNA-150 的改变表达将膀胱癌患者与健康对照者区分开来。lncRNA CASC11 的表达与膀胱癌患者的 miRNA-150 表达呈负相关，但与健康对照组无关。lncRNA CASC11 的过表达介导了 miRNA-150 在癌细胞中的抑制表达，而 miRNA-150 的过表达并没有显著改变 lncRNA CASC11 的表达。lncRNA CASC11 的过表达促进了，而 miRNA-150 的过表达抑制了癌细胞的增殖。miRNA-150 还减弱了 lncRNA CASC11 过表达对癌细胞增殖的增强作用。然而，lncRNA CASC11 的过表达对癌细胞的迁移和侵袭没有显著影响。因此，lncRNA CASC11 可能促进膀胱癌中癌细胞的增殖，lncRNA CASC11 的作用可能是通过 miRNA-150。
