可调谐结构光照明显微镜用于最小处理组织中的背景抑制和成像深度。

Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues.

机构信息

Stanford University, Edward L. Ginzton Laboratory, Stanford, California, United States.

SCREEN Holdings Co., Ltd., R&D Department, Kyoto, Japan.

出版信息

J Biomed Opt. 2019 Apr;24(4):1-6. doi: 10.1117/1.JBO.24.4.046501.

DOI:10.1117/1.JBO.24.4.046501

PMID:30968649

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6454294/

Abstract

We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude.

摘要

我们通过使用可调谐结构照明 (SI) 频率来改善光片荧光显微镜的光学切片，以优化散射样本中的图像质量。使用一维空间光调制器生成相干 SI 图案以获得最大的图案对比度，并且图案空间频率可调整到我们的检测物镜的非相干截止频率的一半。在这个频率下，我们证明了背景降低了 2 个数量级。