Bakas Spyridon, Uttamchandani Deepak, Toshiyoshi Hiroshi, Bauer Ralf
Department of Electronic and Electrical Engineering, University of Strathclyde, Glasgow, UK.
Institute of Industrial Science, University of Tokyo, Tokyo, Japan.
Sci Rep. 2021 Jul 8;11(1):14100. doi: 10.1038/s41598-021-93454-8.
We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 435 × 232 × 60 µm volumes with 0.25 vps (volumes per second) and minimum lateral and axial resolution of 1.0 µm and 3.8 µm respectively. An open-top geometry allows imaging of samples on flat bottomed holders, allowing integration with microfluidic devices, multi-well plates and slide mounted samples, with applications envisaged in biomedical research and pre-clinical settings.
我们设计并实现了一种基于光学微机电系统扫描仪和可调谐透镜的紧凑、经济高效的小型化光片显微镜系统。该系统占地面积为20×28×13厘米，将现成的光学器件和光机械器件与3D打印的结构和光学元件，以及经济实惠的物镜、激发激光器和相机相结合。全光学体积扫描能够以每秒0.25个体积（vps）的速度对435×232×60微米的体积进行成像，横向和轴向分辨率分别为1.0微米和3.8微米。顶部开放式几何结构允许对平底支架上的样品进行成像，从而能够与微流控设备、多孔板和载玻片安装的样品集成，预计在生物医学研究和临床前环境中具有应用前景。
