Mao Feifei, Sun Huiyu, Li Dan, Wang Shengnan, Lu Dan
Beijing DiTan Hospital, Capital Medical University, Beijing, China.
Eur J Ophthalmol. 2020 Jul;30(4):738-742. doi: 10.1177/1120672119841777. Epub 2019 Apr 10.
To determine the value of the polymerase chain reaction analysis of aqueous humor specimens as a tool to diagnose cytomegalovirus retinitis in AIDS patients.
In all, 63 AIDS patients were evaluated in this study. They were sorted into two diagnostic categories: eyes with active cytomegalovirus retinitis and eyes without active cytomegalovirus retinitis. The aqueous humor and blood samples were collected and analyzed by polymerase chain reaction.
A total of 49 patients had active cytomegalovirus retinitis (77.8%) and 14 patients had inactive cytomegalovirus retinitis or normal fundus (22.2%). The mean average of patients was 39 years (range: 22-59). The majority of patients were male (90.5%). Cytomegalovirus DNA was detected in 46 and 7 of 49 aqueous and blood samples, respectively, from AIDS patients with active cytomegalovirus retinitis. We did not detect cytomegalovirus DNA in any of the eyes without active cytomegalovirus retinitis. The sensitivity of polymerase chain reaction in the detection of cytomegalovirus in aqueous humor and blood samples was 93.5% and 14.3%, respectively.
The polymerase chain reaction analysis is a safe, highly specific, and sensitive method to diagnose cytomegalovirus retinitis.
确定对房水样本进行聚合酶链反应分析作为诊断艾滋病患者巨细胞病毒性视网膜炎工具的价值。
本研究共评估了63例艾滋病患者。他们被分为两个诊断类别：患有活动性巨细胞病毒性视网膜炎的眼睛和没有活动性巨细胞病毒性视网膜炎的眼睛。采集房水和血液样本并通过聚合酶链反应进行分析。
共有49例患者患有活动性巨细胞病毒性视网膜炎（77.8%），14例患者患有非活动性巨细胞病毒性视网膜炎或眼底正常（22.2%）。患者的平均年龄为39岁（范围：22 - 59岁）。大多数患者为男性（90.5%）。在患有活动性巨细胞病毒性视网膜炎的艾滋病患者的49份房水和血液样本中，分别有46份和7份检测到巨细胞病毒DNA。在没有活动性巨细胞病毒性视网膜炎的任何眼睛中均未检测到巨细胞病毒DNA。聚合酶链反应检测房水和血液样本中巨细胞病毒的敏感性分别为93.5%和14.3%。
聚合酶链反应分析是诊断巨细胞病毒性视网膜炎的一种安全、高度特异且敏感的方法。
