McCann J D, Margolis T P, Wong M G, Kuppermann B D, Luckie A P, Schwartz D M, Irvine A R, Ai E
Francis I. Proctor Foundation for Research in Ophthalmology, University of California, San Francisco 94122-0944, USA.
Am J Ophthalmol. 1995 Aug;120(2):219-26. doi: 10.1016/s0002-9394(14)72610-8.
To develop a sensitive and specific laboratory assay for the diagnosis of cytomegalovirus retinitis.
We used a polymerase chain reaction-based assay for detection of cytomegalovirus DNA in vitreous samples. We attempted to detect cytomegalovirus DNA in 19 vitreous samples from patients with the acquired immunodeficiency syndrome (AIDS) who had untreated cytomegalovirus retinitis and in 40 vitreous samples from patients with AIDS who had been treated with systemic ganciclovir or foscarnet, or both. We also attempted to detect cytomegalovirus DNA in vitreous samples from 54 immunocompetent patients, including 32 with retinal detachment or macular hole, 11 with vitreous inflammation, and 11 with vitreous hemorrhage. Additionally, we attempted to detect cytomegalovirus DNA in 15 vitreous samples from patients with AIDS who had vitreoretinal inflammation not caused by cytomegalovirus.
Cytomegalovirus DNA was detected in 18 of 19 eyes with untreated cytomegalovirus retinitis. We detected cytomegalovirus DNA in 19 of 40 vitreous samples from patients with previously treated cytomegalovirus retinitis. Cytomegalovirus DNA was not detected in any of 69 patients who did not have a clinical diagnosis of cytomegalovirus retinitis. Thus, the assay had an estimated sensitivity of 95% in detecting untreated cytomegalovirus retinitis and a sensitivity of 48% in detecting cytomegalovirus retinitis that had been treated with systemic ganciclovir or foscarnet, or both. The assay did not give false-positive results in patients with vitreous hemorrhage or vitreous inflammation. Most important, the assay did not give false-positive results in AIDS patients with vitreous inflammation from causes other than cytomegalovirus retinitis.
We have developed a sensitive and specific diagnostic assay that will assist in the diagnosis of cytomegalovirus retinitis.
开发一种用于诊断巨细胞病毒性视网膜炎的灵敏且特异的实验室检测方法。
我们采用基于聚合酶链反应的检测方法来检测玻璃体样本中的巨细胞病毒DNA。我们试图在19份来自患有未经治疗的巨细胞病毒性视网膜炎的获得性免疫缺陷综合征（AIDS）患者的玻璃体样本中，以及40份来自接受过全身更昔洛韦或膦甲酸钠治疗或两者皆用的AIDS患者的玻璃体样本中检测巨细胞病毒DNA。我们还试图在54名免疫功能正常的患者的玻璃体样本中检测巨细胞病毒DNA，其中包括32名患有视网膜脱离或黄斑裂孔的患者、11名患有玻璃体炎症的患者以及11名患有玻璃体出血的患者。此外，我们试图在15份来自患有非巨细胞病毒性视网膜炎引起的玻璃体视网膜炎症的AIDS患者的玻璃体样本中检测巨细胞病毒DNA。
在19只患有未经治疗的巨细胞病毒性视网膜炎的眼中，有18只检测到了巨细胞病毒DNA。我们在40份来自先前接受过治疗的巨细胞病毒性视网膜炎患者的玻璃体样本中，检测到了19份中的巨细胞病毒DNA。在69名没有巨细胞病毒性视网膜炎临床诊断的患者中，均未检测到巨细胞病毒DNA。因此，该检测方法在检测未经治疗的巨细胞病毒性视网膜炎时估计灵敏度为95%，在检测接受过全身更昔洛韦或膦甲酸钠治疗或两者皆用的巨细胞病毒性视网膜炎时灵敏度为48%。该检测方法在玻璃体出血或玻璃体炎症患者中未给出假阳性结果。最重要的是，该检测方法在因非巨细胞病毒性视网膜炎导致玻璃体炎症的AIDS患者中未给出假阳性结果。
我们开发了一种灵敏且特异的诊断检测方法，将有助于巨细胞病毒性视网膜炎的诊断。
