Araújo Fernanda de Paula, Albuquerque Ricardo Diego Duarte Galhardo de, Rangel Leonardo da Silva, Caldas Gabriel Rocha, Tietbohl Luís Armando Cândido, Santos Marcelo Guerra, Ricci-Júnior Eduardo, Thiengo Silvana, Fernandez Monica Ammon, Santos José Augusto Albuquerque Dos, Faria Robson Xavier, Rocha Leandro
Universidade Federal Fluminense, Laboratório de Tecnologia de Produtos Naturais, Niterói, RJ, Brasil.
Universidade Federal Fluminense, Programa de Pós-Graduação em Ciências e Tecnologia, Niterói, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2019 Apr 8;114:e180489. doi: 10.1590/0074-02760180489.
This work describes a chemical study of the essential oil from leaves of Xylopia ochrantha, an endemic Annonaceae species from Brazil, and its activity against Biomphalaria species. Considering its poor solubility in aqueous medium, the essential oil was nanoemulsified to evaluate its action on controlling some mollusc species of genus Biomphalaria, snail hosts of Schistosoma mansoni that causes schistosomiasis, which mainly affects tropical and subtropical countries.
The main aims of this work were to analyse the chemical composition of essential oil from X. ochrantha, and to evaluate the effect of its nanoemulsion on molluscs of genus Biomphalaria and their oviposition.
Chemical analysis was performed by gas chromatography coupled to mass spectrometry. Nanoemulsions were prepared by a low energy method and characterised by particle size and polydispersity index. Biological assays evaluating the mortality of adult species of B. glabrata, B. straminea and B. tenagophila and their ovipositions upon contact with the most stable nanoemulsion during 24 and 48 h were performed.
Chemical analysis by mass spectrometry revealed the majority presence of bicyclogermacrene and germacrene D in the essential oil. The formulation with a hydrophilic-lipophilic balance (HLB) of 9.26 was the most suitable for the oil delivery system. This nanoemulsion caused the mortality in B. tenagophila, B. straminea and B. glabarata of different sizes at levels ranging from 50 to 100% in 48 h. Additionally, the formulation could inhibit the development of deposited eggs.
Thus, these results suggest the use of nanoemulsified essential oil from X. ochrantha as a possible alternative in controlling some Biomphalaria species involved in the schistosomiasis cycle.
本研究描述了对巴西特有的番荔枝科植物奥氏木瓣树叶片精油的化学研究及其对双脐螺属物种的活性。鉴于该精油在水介质中的溶解度较差，将其制成纳米乳剂以评估其对一些双脐螺属软体动物的控制作用，这些双脐螺是导致血吸虫病的曼氏血吸虫的中间宿主，血吸虫病主要影响热带和亚热带国家。
本研究的主要目的是分析奥氏木瓣树精油的化学成分，并评估其纳米乳剂对双脐螺属软体动物及其产卵的影响。
采用气相色谱 - 质谱联用进行化学分析。通过低能法制备纳米乳剂，并通过粒径和多分散指数进行表征。进行生物测定，评估光滑双脐螺、稻草双脐螺和嗜栖双脐螺成体在与最稳定的纳米乳剂接触24小时和48小时后的死亡率及其产卵情况。
质谱化学分析表明，精油中主要存在双环吉马烯和吉马烯D。亲水亲油平衡（HLB）为9.26的配方最适合该油递送系统。这种纳米乳剂在48小时内导致不同大小的嗜栖双脐螺、稻草双脐螺和光滑双脐螺的死亡率在50%至100%之间。此外，该配方可抑制所产卵子的发育。
因此，这些结果表明，奥氏木瓣树纳米乳化精油可作为控制血吸虫病传播周期中某些双脐螺属物种的一种可能替代方法。
