Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Hofsvallagata 53, IS-107 Reykjavík, Iceland.
Department of Material and Commodity Sciences and Textile Metrology, Lodz University of Technology, 90-924 Lodz, Poland.
Int J Mol Sci. 2019 Apr 9;20(7):1743. doi: 10.3390/ijms20071743.
,,-trimethyl chitosan (TMC) with 93% degree of trimethylation was synthesized. TMC and the chitosan starting material were subjected to acidic hydrolysis to produce 49 different samples with a reduced average molecular weight (M) ranging from 2 to 144 kDa. This was done to allow the investigation of the relationship between antibacterial activity and M over a wide M range. NMR investigation showed that hydrolysis did not affect the degree of trimethylation (DS) or the structure of the polymer backbone. The activity of TMC against () increased sharply with M until a certain M value (critical M for high activity, CMW) was reached. After the CMW, the activity was not affected by a further increase in the M. A similar pattern of activity was observed for chitosan. The CMW was determined to be 20 kDa for TMC and 50 kDa for chitosan.
，-三甲基壳聚糖（TMC），其三甲基化程度为 93%。TMC 和壳聚糖起始材料都进行了酸水解，产生了 49 个不同的样品，其平均分子量（M）从 2 到 144 kDa 不等。这是为了允许在很宽的 M 范围内研究抗菌活性与 M 之间的关系。NMR 研究表明，水解不影响三甲基化程度（DS）或聚合物主链的结构。TMC 对（）的活性随着 M 的增加而急剧增加，直到达到一定的 M 值（高活性的临界 M，CMW）。在 CMW 之后，M 的进一步增加不会影响活性。壳聚糖也观察到了类似的活性模式。CMW 被确定为 TMC 的 20 kDa 和壳聚糖的 50 kDa。
