实验感染野生啮齿动物中鼠疫抗原和抗体检测的比较试验。
Comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents.
作者信息
Shepherd A J, Hummitzsch D E, Leman P A, Swanepoel R, Searle L A
出版信息
J Clin Microbiol. 1986 Dec;24(6):1075-8. doi: 10.1128/jcm.24.6.1075-1078.1986.
The enzyme-linked immunosorbent assay (ELISA) was compared with other standard tests for detection of plague (Yersinia pestis) antibody and antigen in multimammate mice (Mastomys coucha and M. natalensis) which were experimentally infected and then killed at daily intervals postinoculation. For detection of antibody in sera from M. natalensis, the immunoglobulin G (IgG) ELISA was equivalent in sensitivity to passive hemagglutination and more sensitive than the IgM ELISA and complement fixation. Antibody was first detected on postinfection day 6 by all four tests, but IgM ELISA titers had declined to undetectable levels after 8 weeks. For detection of fraction 1 Y. pestis antigen in rodent organs, the ELISA was less sensitive than fluorescent antibody but more sensitive than complement fixation or immunodiffusion. Plague fraction 1 antigen was detected in 16 of 34 bacteremic sera from M. coucha and M. natalensis. The threshold sensitivity of the ELISA was approximately 10(5) Y. pestis per ml.
将酶联免疫吸附测定（ELISA）与其他标准检测方法进行比较，以检测多乳鼠（非洲沼鼠和纳塔尔多乳鼠）体内的鼠疫（耶尔森氏鼠疫杆菌）抗体和抗原。这些多乳鼠经实验感染后，在接种后的每天间隔时间处死。对于检测纳塔尔多乳鼠血清中的抗体，免疫球蛋白G（IgG）ELISA的敏感性与被动血凝试验相当，且比IgM ELISA和补体结合试验更敏感。所有四种检测方法均在感染后第6天首次检测到抗体，但8周后IgM ELISA滴度已降至无法检测的水平。对于检测啮齿动物器官中的鼠疫杆菌1号抗原，ELISA的敏感性低于荧光抗体法，但高于补体结合试验或免疫扩散法。在34份来自非洲沼鼠和纳塔尔多乳鼠的菌血症血清中，有16份检测到了鼠疫杆菌1号抗原。ELISA的阈值敏感性约为每毫升10(5)个鼠疫杆菌。
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