在急性鼠疫期间使用酶联免疫吸附测定法测量抗原血症。
Use of an enzyme-linked immunosorbent assay to measure antigenaemia during acute plague.
作者信息
Williams J E, Gentry M K, Braden C A, Leister F, Yolken R H
出版信息
Bull World Health Organ. 1984;62(3):463-6.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed to measure concentrations of the specific F1 antigen of the plague bacillus in biological fluids. The assay employed a monoclonal antibody to capture the antigen. Sensitivity of the assay was 0.4 ng of F1 antigen. ELISA-inhibition was used to confirm the specificity of the reactions.This assay detected F1 antigen in two of ten sera from patients with acute bubonic plague and indicated that antigenaemia in man during plague may reach levels of 4-8 mug of F1 antigen per ml of serum.The probability for a correct serodiagnosis of plague was improved when the patients' sera were tested for both antibody and antigen. Two patients with antigenaemia did not have antibody, while two patients with antibody lacked antigenaemia.
摘要
开发了一种酶联免疫吸附测定法（ELISA）来测量生物体液中鼠疫杆菌特异性F1抗原的浓度。该测定法使用单克隆抗体捕获抗原。该测定法的灵敏度为0.4纳克F1抗原。采用ELISA抑制法确认反应的特异性。该测定法在10例急性腺鼠疫患者的血清中检测到2例F1抗原，并表明鼠疫期间人体中的抗原血症水平可能达到每毫升血清4-8微克F1抗原。当检测患者血清中的抗体和抗原时，鼠疫血清学诊断正确的概率提高。两名有抗原血症的患者没有抗体，而两名有抗体的患者没有抗原血症。
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