Shepherd A J, Leman P A, Hummitzsch D E, Swanepoel R
Trans R Soc Trop Med Hyg. 1984;78(6):771-3. doi: 10.1016/0035-9203(84)90014-2.
Sera collected from 691 dogs and 231 rodents in South Africa were tested in parallel by the passive haemagglutination (PHA) and enzyme-linked immunosorbent assay (ELISA) for antibodies to the Fraction 1 antigen of the plague bacillus (Yersinia pseudotuberculosis subsp. pestis). There was a high degree of correlation between the tests, both in over-all numbers positive and in serum titres. 42 dog sera were positive by PHA and 41 by ELISA, 40 sera being positive by both tests. However, using ELISA the numbers of non-specific reactors in dog sera were cut by almost two thirds compared to PHA. The results of tests on rodent sera were identical: only one rodent was positive in both tests. The findings suggest that the ELISA test may have advantages over PHA for large scale serological surveillance.
对从南非收集的691份犬血清和231份啮齿动物血清，采用被动血凝试验（PHA）和酶联免疫吸附测定（ELISA）同时检测鼠疫杆菌（假结核耶尔森菌鼠疫亚种）1号抗原的抗体。在总体阳性数量和血清滴度方面，两种检测方法之间都存在高度相关性。PHA检测出42份犬血清呈阳性，ELISA检测出41份呈阳性，两种检测均呈阳性的血清有40份。然而，与PHA相比，使用ELISA时犬血清中非特异性反应的数量减少了近三分之二。对啮齿动物血清的检测结果相同：两种检测中只有一只啮齿动物呈阳性。这些发现表明，在大规模血清学监测中，ELISA检测可能比PHA更具优势。
