Ha-Kawa K, Hara J, Keiko Y, Muraguchi A, Kawamura N, Ishihara S, Doi S, Yabuuchi H
J Clin Invest. 1986 Dec;78(6):1439-42. doi: 10.1172/JCI112733.
We describe a 10-yr-old boy with T-lineage non-Hodgkin's lymphoma. He had a mediastinal mass, swollen supraclavicular lymph nodes, and pleural effusion. A supraclavicular lymph node biopsy under light microscopy showed a malignant lymphoma of diffuse lymphoblastic type. Most of the cells taken from the malignant pleural effusion expressed T cell-associated antigens such as Leu-1 and OKT 8. To confirm these antigens as T-lineage lymphoma, we examined genomic DNA from malignant cells obtained from the pleural effusion. As was expected, T cell receptor beta-chain gene rearrangements were demonstrated. However, when the immunoglobulin gene organization was analyzed, we detected rearrangements in both the heavy- and kappa-chain genes. To our knowledge, this is the first case in which kappa-chain gene rearrangement was detected in apparent T-lineage cells. These findings provide important information relating to determination of the cellular lineage of lymphoid malignancy.
我们描述了一名患有T细胞系非霍奇金淋巴瘤的10岁男孩。他有纵隔肿块、锁骨上淋巴结肿大及胸腔积液。锁骨上淋巴结活检的光镜检查显示为弥漫性淋巴细胞型恶性淋巴瘤。从恶性胸腔积液中获取的大多数细胞表达T细胞相关抗原，如Leu-1和OKT 8。为了确认这些抗原为T细胞系淋巴瘤，我们检测了从胸腔积液中获取的恶性细胞的基因组DNA。正如预期的那样，证实了T细胞受体β链基因重排。然而，当分析免疫球蛋白基因结构时，我们在重链和κ链基因中均检测到重排。据我们所知，这是首例在明显的T细胞系细胞中检测到κ链基因重排的病例。这些发现为确定淋巴恶性肿瘤的细胞系提供了重要信息。
