Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194, Japan.
Mediators Inflamm. 2010;2010:804298. doi: 10.1155/2010/804298. Epub 2010 Dec 28.
Keishibukuryogan (KBG) is one of the traditional herbal formulations widely administered to patients with blood stagnation for improving blood circulation; currently, it is the most frequently prescribed medicine in Japan. KBG has been reported to improve conjunctional microcirculation. The aim of this study was to evaluate the role of KBG and paeoniflorin, a bioactive compound of KBG, in inhibiting the production of inflammatory cytokines using human dermal microvessel endothelial cells (HDMECs). The authors observed that lipopolysaccharide (LPS; 1 μg/mL) stimulated the secretion of proinflammatory cytokines in HDMECs. KBG treatment (10 mg/mL) significantly suppressed the mRNA levels of migration inhibitory factor (MIF), interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α in LPS-stimulated cultured HDMECs. Similarly, paeoniflorin significantly suppressed the mRNA levels of these cytokines in LPS-stimulated cultured HDMECs. ELISA showed that KBG and paeoniflorin suppressed the production of MIF, IL-6, IL-8, and TNF-α in LPS-stimulated HDMECs. Moreover, KBG and paeoniflorin decreased the expression of cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) in these cells. These results suggest that KBG may be useful for improving microvascular inflammation in patients with skin diseases.
葛根汤是一种常用于改善血液循环的传统草药配方，目前在日本是最常被开的处方药物。葛根汤已被报道能改善结合微脉管循环。本研究旨在评估葛根汤及其生物活性化合物芍药苷在抑制人真皮微血管内皮细胞（HDMEC）中炎症细胞因子产生中的作用。作者观察到脂多糖（LPS；1μg/mL）刺激 HDMEC 中促炎细胞因子的分泌。葛根汤（10mg/mL）处理显著抑制 LPS 刺激培养的 HDMECs 中迁移抑制因子（MIF）、白细胞介素（IL）-6、IL-8 和肿瘤坏死因子（TNF）-α的 mRNA 水平。同样，芍药苷也显著抑制 LPS 刺激培养的 HDMECs 中这些细胞因子的 mRNA 水平。ELISA 显示，葛根汤和芍药苷抑制 LPS 刺激的 HDMECs 中 MIF、IL-6、IL-8 和 TNF-α的产生。此外，葛根汤和芍药苷降低了这些细胞中环氧化酶-2 和诱导型一氧化氮合酶（iNOS）的表达。这些结果表明，葛根汤可能有助于改善皮肤病患者的微血管炎症。
