Yan Miao, Vemu Bhaskar, Veenstra Jacob, Petiwala Sakina M, Johnson Jeremy J
Department of Pharmacy Practice, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Institute of Clinical Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Integr Mol Med. 2018 Aug;5(4). doi: 10.15761/IMM.1000335. Epub 2018 Aug 15.
Rosemary is abundant with phytochemicals and has recently been approved as an antioxidant food preservative in the European Union. The safety of rosemary is well established, however, the benefits on gastrointestinal health are less known. Our overall hypothesis is that the phytochemicals in rosemary including carnosol have the potential to promote gastrointestinal health by activation of the antioxidant sestrin-2 when consumed in our diet.
Colon cells HCT116 and SW480 were treated with carnosol and evaluated by MTT, immunofluorescence, ELISA, and Western blot analysis to understand the modulation of the PERK/Nrf2/Sestrin-2 pathway.
Carnosol was found to modulate PERK and increase the concentration of nuclear Nrf2. Furthermore, a downstream marker of Nrf2 expression, Sestrin-2 was shown to be upregulated.
Based on these observations carnosol modulates the PERK and Nrf2 pathways along with increased expression of sestrin-2, a known stress inducible antioxidant.
迷迭香富含植物化学物质，最近在欧盟被批准作为抗氧化食品防腐剂。迷迭香的安全性已得到充分证实，然而，其对胃肠道健康的益处却鲜为人知。我们的总体假设是，迷迭香中的植物化学物质，包括鼠尾草酚，在饮食中摄入时，有可能通过激活抗氧化蛋白 sestrin - 2来促进胃肠道健康。
用鼠尾草酚处理结肠细胞HCT116和SW480，并通过MTT、免疫荧光、ELISA和蛋白质印迹分析进行评估，以了解PERK/Nrf2/Sestrin - 2通路的调节情况。
发现鼠尾草酚可调节PERK并增加核Nrf2的浓度。此外，Nrf2表达的下游标志物Sestrin - 2被证明上调。
基于这些观察结果，鼠尾草酚可调节PERK和Nrf2通路，同时增加sestrin - 2的表达，sestrin - 2是一种已知的应激诱导抗氧化剂。
