Tong Xiao-Pei, Ma Yan-Xia, Quan Dan-Ni, Zhang Ling, Yan Miao, Fan Xin-Rong
Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province 410011, China.
Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China.
Evid Based Complement Alternat Med. 2017;2017:7359806. doi: 10.1155/2017/7359806. Epub 2017 Feb 13.
In the past few decades, the incidence of liver cancer has been rapidly rising across the world. Rosemary is known to possess antioxidant activity and is used as natural antioxidant food preservative. It is proposed to have anticancer activity in treating different tumor models. In this study, we try to explore the impact of rosemary extracts on upregulating the level of Nrf2 and Nrf2-regulatory proteins, Sestrin2 and MRP2 in HepG2 cells, and to speculate its potential mechanism. The anticancer activity of rosemary extract, including its polyphenolic diterpenes carnosic acid and carnosol, was evaluated to understand the potential effect on HepG2 cells. Rosemary extract, carnosic acid, and carnosol induced the expression of Sestrin2 and MRP2 associate with enhancement of Nrf2 protein level in HepG2 cells, in which carnosic acid showed most obvious effect. Although the activation pathway of Nrf2/ARE was not exactly assessed, it can be assumed that the enhancement of expression of Sestrin2 and MRP2 may result from upregulation of Nrf2.
在过去几十年中，全球肝癌发病率一直在迅速上升。迷迭香具有抗氧化活性，被用作天然抗氧化食品防腐剂。据推测，它在治疗不同肿瘤模型方面具有抗癌活性。在本研究中，我们试图探讨迷迭香提取物对上调HepG2细胞中Nrf2以及Nrf2调节蛋白Sestrin2和MRP2水平的影响，并推测其潜在机制。评估了迷迭香提取物（包括其多酚二萜类成分鼠尾草酸和鼠尾草酚）的抗癌活性，以了解其对HepG2细胞的潜在作用。迷迭香提取物、鼠尾草酸和鼠尾草酚诱导了HepG2细胞中Sestrin2和MRP2的表达，这与Nrf2蛋白水平的提高相关，其中鼠尾草酸的作用最为明显。尽管未确切评估Nrf2/ARE的激活途径，但可以推测Sestrin2和MRP2表达的增强可能是由于Nrf2的上调所致。
