Dobrucka Renata, Kaczmarek Mariusz, Łagiedo Małgorzata, Kielan Agata, Dlugaszewska Jolanta
Department of Industrial Products Quality and Ecology, Faculty of Commodity Science, Poznań University of Economics and Business, al. Niepodległości 10, 61-875 Poznan, Poland.
Department of Immunology, Chair of Clinical Immunology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland.
Saudi Pharm J. 2019 Mar;27(3):373-383. doi: 10.1016/j.jsps.2018.12.006. Epub 2018 Dec 17.
Due to the search for new methods of producing bimetallic nanoparticles, in this work, we have conducted a biological synthesis of Au-CuO and CuO-ZnO nanoparticles using . The synthesized Au-CuO and CuO-ZnO nanoparticles were also analyzed in terms of their antibacterial activity, as well as their influence on cell viability, using two specific cell lines: C6 rat brain glioma (ATCC® CCL-107™) and T98G human glioma (ATCC® CRL-1690™). The studies carried out by means of Atomic Force Microscopy helped to determine the presence Au-CuO nanoparticles whose size was about 13 nm. The size of CuO-ZnO nanoparticles was about 28 nm. The obtained nanoparticles showed cidal activity against glioma cells depending on the concentration of the substance and the time of culture. In the first stage, the nanoparticles limited the ability to divide cells; then, they blocked the cell cycle in the G2 - M phase, and finally led to massive cell death. The antimicrobial activity studies showed that Au-CuO nanoparticles inhibited the growth of microorganisms at lower concentrations than CuO-ZnO nanoparticles, and both kinds of nanoparticles showed excellent cidal properties.
由于在寻找生产双金属纳米颗粒的新方法，在这项工作中，我们使用……进行了金-氧化铜和氧化铜-氧化锌纳米颗粒的生物合成。还使用两种特定细胞系：C6大鼠脑胶质瘤细胞（ATCC® CCL-107™）和T98G人胶质瘤细胞（ATCC® CRL-1690™），对合成的金-氧化铜和氧化铜-氧化锌纳米颗粒的抗菌活性及其对细胞活力的影响进行了分析。通过原子力显微镜进行的研究有助于确定存在尺寸约为13纳米的金-氧化铜纳米颗粒。氧化铜-氧化锌纳米颗粒的尺寸约为28纳米。所获得的纳米颗粒根据物质浓度和培养时间对胶质瘤细胞表现出杀菌活性。在第一阶段，纳米颗粒限制了细胞分裂能力；然后，它们将细胞周期阻断在G2 - M期，最终导致大量细胞死亡。抗菌活性研究表明，金-氧化铜纳米颗粒在比氧化铜-氧化锌纳米颗粒更低的浓度下就能抑制微生物生长，并且两种纳米颗粒都表现出优异的杀菌性能。
