Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Pulau Pinang, Malaysia.
Ann Joo Integrated Steel Sdn Bhd, Lot 1236, Prai Industrial Estate, 13600, Prai, Penang, Malaysia.
Sci Rep. 2021 Jan 8;11(1):99. doi: 10.1038/s41598-020-79547-w.
Calotropis gigantea (C. gigantea) extract with an ecofriendly nanotechnology approach could provide promising antimicrobial activity against skin pathogens. This study investigates the antimicrobial capability of green synthesized binary ZnO-CuO nanocomposites from C. gigantea against non-MDR (Staphylococcus aureus and Escherichia coli) and MDR (Klebsiella pneumoniae, Pseudomonas aeruginosa and methicillin-resistant S. aureus) skin pathogens. Scanning electron microscopy and transmission electron microscopy revealed the size and shape of B3Z1C sample. Results of X-ray powder diffraction, energy-dispersive spectroscopy, FTIR and UV-Vis spectroscopy analyses confirmed the presence of mixed nanoparticles (i.e., zinc oxide, copper oxide, carbon and calcium) and the stabilising phytochemical agents of plant (i.e., phenol and carbonyl). Antimicrobial results showed that carbon and calcium decorated binary ZnO-CuO nanocomposites with compositions of 75 wt% of ZnO and 25 wt% CuO (B3Z1C) was a strong bactericidal agent with the MBC/MIC ratio of ≤ 4 and ≤ 2 for non-MDR and MDR pathogens, respectively. A significant non-MDR zone of inhibitions were observed for BZC by Kirby-Bauer disc-diffusion test. Further time-kill observation revealed significant fourfold reduction in non-MDR pathogen viable count after 12 h study period. Further molecular studies are needed to explain the biocidal mechanism underlying B3Z1C potential.
采用环保纳米技术的白花鬼针草提取物可能对皮肤病原体具有有前景的抗菌活性。本研究调查了由白花鬼针草绿色合成的二元 ZnO-CuO 纳米复合材料对非耐多药（金黄色葡萄球菌和大肠杆菌）和耐多药（肺炎克雷伯菌、铜绿假单胞菌和耐甲氧西林金黄色葡萄球菌）皮肤病原体的抗菌能力。扫描电子显微镜和透射电子显微镜显示了 B3Z1C 样品的尺寸和形状。X 射线粉末衍射、能量色散光谱、傅里叶变换红外光谱和紫外-可见光谱分析的结果证实了混合纳米颗粒（即氧化锌、氧化铜、碳和钙）和植物稳定化植物化学物质（即酚类和羰基）的存在。抗菌结果表明，碳和钙修饰的二元 ZnO-CuO 纳米复合材料中 ZnO 的重量百分比为 75%，CuO 的重量百分比为 25%（B3Z1C）是一种强杀菌剂，对非耐多药和耐多药病原体的 MBC/MIC 比值分别为 ≤4 和 ≤2。Kirby-Bauer 圆盘扩散试验观察到 BZC 对非耐多药病原体具有显著的抑菌区。进一步的时间杀伤观察显示，非耐多药病原体的活菌数在 12 小时研究期后减少了四倍。需要进一步的分子研究来解释 B3Z1C 潜在的杀菌机制。
