厄瓜多尔32型序列中的新德里金属β-内酰胺酶-1（NDM-1）碳青霉烯酶
NDM-1 carbapenemase in sequence type 32 in Ecuador.
作者信息
Villacís J E, Bovera M, Romero-Alvarez D, Cornejo F, Albán V, Trueba G, Dorn H F, Reyes J A
机构信息
Carrera de Bioquímica Clínica, Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador.
Centro de Referencia Nacional de Resistencia a los Antimicrobianos Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Quito, Ecuador.
出版信息
New Microbes New Infect. 2019 Mar 8;29:100526. doi: 10.1016/j.nmni.2019.100526. eCollection 2019 May.
Abstract
OBJECTIVES
To describe a clinical case of sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador.
METHODS
We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an isolate. We used synergy with the imipenem-EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing.
RESULTS
Molecular characterization revealed the presence of . ST32 harbouring the gene in Ecuador. The gene was isolated through PCR and amplified from a purified plasmid.
CONCLUSIONS
To the best of our knowledge, this is the first report of . ST32 harbouring the gene.
摘要
目的
描述在厄瓜多尔发现的携带新德里金属β-内酰胺酶（NDM）的序列类型（ST）32的临床病例。
方法
我们使用多位点序列分型（MLST）来确认分离株的细菌种类和序列类型。我们采用亚胺培南-EDTA纸片协同法和碳青霉烯酶灭活法（CIM）来确定碳青霉烯酶的产生；通过PCR扩增和扩增子测序确认碳青霉烯酶基因的存在。
结果
分子特征显示在厄瓜多尔存在携带该基因的ST32。该基因通过PCR从纯化质粒中分离并扩增得到。
结论
据我们所知，这是首次报道携带该基因的ST32。
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