Kpemissi Mabozou, Eklu-Gadegbeku Kwashie, Veerapur Veeresh P, Potârniche Adrian-Valentin, Adi Kodjo, Vijayakumar S, Banakar Siddalingesh M, Thimmaiah N V, Metowogo Kossi, Aklikokou Kodjo
Faculty of Sciences, University of Lomé, Togo.
University of Agricultural Science and Veterinary Medicine, Manastur Street. 3-5, 400372, Cluj-Napoca, Romania.
Heliyon. 2019 Mar 28;5(3):e01365. doi: 10.1016/j.heliyon.2019.e01365. eCollection 2019 Mar.
Management of chronic renal failure is exceedingly expensive. Despite of encouraging experimental outcomes, there is a lack of potent nephroprotective drugable molecules in a clinics or market. To develop a nephroprotective phytomedicine, the present study was designed to do a literature survey on reported phytochemical and biological analysis of and to carry out chemoprofiling, in-vitro antioxidant and ex-vivo nephroprotective capacity of the title plant. The phytochemical and biological activity survey of has reveals the presence of many bioactive compounds such as flavonoids, terpenoids, steroids and alkaloids with many biological activities. Phytochemical investigation re-confirmed the presence of these compounds. Hydroalcoholic extract of (CM extract) showed a strong antioxidant activity by scavenging AAPH, DPPH, nitric oxide, hydrogen peroxide and chelating metal ions. CM extract exhibited significant ( < 0.001) dose dependent inhibition of ferric chloride-ascorbic acid induced lipid peroxidation. Diabetic nephropathy is a serious and common complication leading to end stage renal disease. Therefore, in the present study, glucose-induced toxicity was also studied in human embryonic kidney cells (HEK-293) as an in vitro model for diabetic nephropathy. The results showed that exposure of cells to high glucose (100 mM) for 72 h significantly reduced the cell viability resulting in morphological changes such as cell shrinkage, rounded cell shape and cytoplasmic vacuolation. Treatment with CM extract at 10 and 25 μg/mL resulted in significant improvement in cell viability from 10 to 23% compared to the high glucose control. This study demonstrated the potential antioxidant and nephroprotective properties of , justifying its traditional use in the treatment of various diseases.
慢性肾衰竭的治疗费用极其高昂。尽管实验结果令人鼓舞，但临床上或市场上缺乏有效的具有肾保护作用的可成药分子。为了开发一种具有肾保护作用的植物药，本研究旨在对已报道的某植物的植物化学和生物学分析进行文献综述，并对该植物进行化学谱分析、体外抗氧化和体内肾保护能力研究。对该植物的植物化学和生物活性调查揭示了许多生物活性化合物的存在，如黄酮类、萜类、甾体类和生物碱类，具有多种生物活性。植物化学研究再次证实了这些化合物的存在。该植物的水醇提取物（CM提取物）通过清除偶氮二异丁脒盐酸盐（AAPH）、二苯基苦味酰基自由基（DPPH）、一氧化氮、过氧化氢和螯合金属离子表现出强大的抗氧化活性。CM提取物对氯化铁-抗坏血酸诱导的脂质过氧化表现出显著的（P<0.001）剂量依赖性抑制作用。糖尿病肾病是导致终末期肾病的一种严重且常见的并发症。因此，在本研究中，还以人胚肾细胞（HEK-293）作为糖尿病肾病的体外模型研究了葡萄糖诱导的毒性。结果表明，将细胞暴露于高糖（100 mM）72小时会显著降低细胞活力，导致细胞形态发生变化，如细胞收缩、细胞形状变圆和细胞质空泡化。与高糖对照组相比，用10和25μg/mL的CM提取物处理可使细胞活力显著提高10%至23%。本研究证明了该植物具有潜在的抗氧化和肾保护特性，证明了其在治疗各种疾病中的传统用途。
