Amare Yosef Eshetie, Dires Kassahun, Asfaw Tsegahun
Department of Biomedical Sciences, Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia.
Department of Pharmacy, Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia.
Evid Based Complement Alternat Med. 2022 Oct 26;2022:6990263. doi: 10.1155/2022/6990263. eCollection 2022.
Despite the development of oral hypoglycemic medications, diabetes and its associated complications continue to be significant clinical issues. The purpose of this study was to examine the antidiabetic effects of (L.) Wilczek seeds in mice that had been given alloxan to cause diabetes.
In Swiss albino mice, diabetes was brought on by a single intraperitoneal injection of the drug alloxan (150 mg/kg). For 14 days, glibenclamide (5 mg/kg) and methanol extract of seeds (100, 200, and 400 mg/kg) were given orally. Following oral administration of to mice, the blood glucose levels (BGL) and body weight were measured at 7 and 14 days. The mice were sacrificed at the end of the trial, and blood samples were taken for the evaluation of insulin, glycated hemoglobin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), high-density lipoprotein (HDL), total cholesterol (TC), and triglyceride (TG) levels. It was determined how much glycogen was present in the liver. Additionally, the total phenolic and flavonoid contents of were determined, along with the in vitro DPPH (2, 2 diphenyl-1-picrylhrazyl) free radical-scavenging activity. < 0.05 was chosen as the cutoff for statistical significance.
Following oral administration of for 14 days, diabetic mice's BGL and bad cholesterol (TC and TG) levels significantly decreased, while HDL levels increased. Treatment with significantly decreased the levels of AST, ALT, and glycated hemoglobin when compared with diabetes control. On the other hand, it raised insulin levels and the amount of liver glycogen. underwent phytochemical analysis, which identified the presence of tannins, saponins, phenols, alkaloids, terpenoids, steroids, flavonoids, and glycosides. Per gram of seed extract, the total phenolic content was 43.12 ± 3.14 mg of gallic acid equivalents, while the total flavonoid content was 38.35 ± 2.6 mg of quercetin equivalents. Ascorbic acid was shown to have an IC value of 18.64 g/ml during a DPPH-scavenging assay, while had an IC value of 73.35 g/ml.
According to the findings of the current study, the methanolic extract of the seeds from the plant possesses significant antidiabetic characteristics that are on par with those of the commonly used drug glibenclamide. Hence, seems to be effective as a natural antidiabetic.
尽管口服降糖药物有所发展，但糖尿病及其相关并发症仍然是重大的临床问题。本研究的目的是检测**（植物名称未给出，原文此处为空白）**威尔克泽克种子对用四氧嘧啶诱导糖尿病的小鼠的抗糖尿病作用。
在瑞士白化小鼠中，通过单次腹腔注射四氧嘧啶（150mg/kg）诱导糖尿病。连续14天口服给予格列本脲（5mg/kg）和**（植物名称未给出，原文此处为空白）种子的甲醇提取物（100、200和400mg/kg）。给小鼠口服（植物名称未给出，原文此处为空白）后，在第7天和第14天测量血糖水平（BGL）和体重。试验结束时处死小鼠，采集血样以评估胰岛素、糖化血红蛋白、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、高密度脂蛋白（HDL）、总胆固醇（TC）和甘油三酯（TG）水平。测定肝脏中糖原的含量。此外，还测定了（植物名称未给出，原文此处为空白）**的总酚和黄酮含量，以及体外DPPH（2,2-二苯基-1-苦基肼）自由基清除活性。选择P<0.05作为统计学显著性的临界值。
口服**（植物名称未给出，原文此处为空白）14天后，糖尿病小鼠的血糖水平和坏胆固醇（TC和TG）水平显著降低，而HDL水平升高。与糖尿病对照组相比，（植物名称未给出，原文此处为空白）治疗显著降低了AST、ALT和糖化血红蛋白水平。另一方面，它提高了胰岛素水平和肝糖原含量。对（植物名称未给出，原文此处为空白）进行了植物化学分析，确定其含有单宁、皂苷、酚类、生物碱、萜类、甾体、黄酮类和糖苷。每克（植物名称未给出，原文此处为空白）种子提取物的总酚含量为43.12±3.14mg没食子酸当量，总黄酮含量为38.35±2.6mg槲皮素当量。在DPPH清除试验中，抗坏血酸的IC值为18.64μg/ml，而（植物名称未给出，原文此处为空白）**的IC值为73.35μg/ml。
根据本研究结果，**（植物名称未给出，原文此处为空白）植物种子的甲醇提取物具有显著的抗糖尿病特性，与常用药物格列本脲相当。因此，（植物名称未给出，原文此处为空白）**似乎是一种有效的天然抗糖尿病药物。
