Wibowo Risky Hadi, Darwis Welly, Yudha Salprima, Purnaningsih Ismu, Siboro Resli
Faculty of Mathematics and Natural Science, University of Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38122, Indonesia.
Master Program of Biology, Faculty of Mathematics and Natural Science, University of Bengkulu, Jl. W.R Supratman, Kandang Limun, Bengkulu 38122, Indonesia.
Int J Microbiol. 2022 Nov 16;2022:6435202. doi: 10.1155/2022/6435202. eCollection 2022.
Exploration studies of endophytic bacteria from (L.) and their potential have not much been conducted. This research aims to explore and characterize the antimicrobial activity of endophytic bacteria in against pathogenic bacteria. This research consists of several steps including the isolation of bacteria, screening of the antimicrobial activity assay using the dual cross streak method, molecular identification through 16s rDNA analysis, and characterization of bioactive compound production through PKS-NRPS gene detection and GC-MS analysis. There are 29 endophytic bacteria that were successfully isolated from . The antimicrobial activity showed that there are four potential isolates AKEBG21, AKEBG23, AKEBG25, and AKEBG28 that can inhibit the growth of pathogenic bacteria such as and . The 16S rDNA sequence analysis showed that these isolates are identified as . These four isolates are identified as able to produce the bioactive compounds through the detection of polyketide synthase (PKS) and nonribosomal peptide synthase (NRPS)-encoding genes. AKEBG23 has the highest inhibition against pathogenic bacteria, and according to the GC-MS analysis, five major compounds are allegedly involved in its antimicrobial activity such as butylated hydroxytoluene (BHT), diisooctyl phthalate, E-15-heptadecenal, 1-heneicosanol, and E-14-hexadecenal. This result suggested that AKEBG23 as the endophytic bacterium from has a beneficial role as well as the plant itself. The bacterium produces several bioactive compounds that are allegedly involved in its antimicrobial activity against pathogenic bacteria.
对[植物名称]（此处原文植物名称缺失）内生细菌的探索性研究及其潜力尚未得到充分开展。本研究旨在探索和表征[植物名称]内生细菌对病原菌的抗菌活性。本研究包括几个步骤，包括细菌分离、使用双交叉划线法进行抗菌活性测定筛选、通过16s rDNA分析进行分子鉴定，以及通过聚酮合酶-非核糖体肽合成酶（PKS-NRPS）基因检测和气相色谱-质谱联用（GC-MS）分析来表征生物活性化合物的产生。从[植物名称]中成功分离出29株内生细菌。抗菌活性表明，有四个潜在菌株AKEBG21、AKEBG23、AKEBG25和AKEBG28能够抑制诸如[病原菌名称1]和[病原菌名称2]（此处原文病原菌名称缺失）等病原菌的生长。16S rDNA序列分析表明，这些菌株被鉴定为[具体菌种名称]（此处原文具体菌种名称缺失）。通过检测编码聚酮合酶（PKS）和非核糖体肽合成酶（NRPS）的基因，确定这四个菌株能够产生生物活性化合物。AKEBG23对病原菌的抑制作用最强，根据GC-MS分析，据称有五种主要化合物参与其抗菌活性，如丁基化羟基甲苯（BHT）、邻苯二甲酸二异辛酯、E-15-十七碳烯醛、1-二十一烷醇和E-14-十六碳烯醛。这一结果表明，作为来自[植物名称]的内生细菌，AKEBG23与植物本身一样具有有益作用。该细菌产生几种据称参与其对病原菌抗菌活性的生物活性化合物。
