Selvakumar Jemimah Naine, Chandrasekaran Subathra Devi, Vaithilingam Mohanasrinivasan
Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
Pharmacogn Mag. 2015 Oct;11(Suppl 3):S469-73. doi: 10.4103/0973-1296.168974.
Recently, numerous pathogens have developed resistance due to the indiscriminate use of commercial therapeutic drugs.
The main aim of the study was to evaluate the bioactive potential of the Streptomyces spectabilis VITJS10 crude extract.
The S. spectabilis VITJS10 ethyl acetate extract was tested for antibacterial, antioxidant, and cytotoxic properties. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical constitutes were determined by gas chromatography-mass spectrometry (GC-MS).
The antibacterial potential revealed the effective activity against Shigellaflexneri (MTCC No: 1457) (22 mm), Salmonella typhi (MTCC No: 1167) (23 mm), Escherichia coli (MTCC No: 1588) (22 mm), Pseudomonas aeruginosa (MTCC No: 4676) (22 mm) at 20 mg/mL concentration. Scavenging ability of the extract was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay revealing its 95% inhibition at 5 mg/mL concentration. Hepatocellular cancer cells (HepG2) cell line was used to evaluate the cytotoxicity by 3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide assay. The extract showed maximum inhibition at IC50 of 250 μg/mL with 53.6% cell viability. The highest 16S rRNA gene sequence homogeneity was observed 99% similar with the novel strain S. spectabilis S3-1. The chemical components of the crude extract of VITJS10 were detected with 37 chemical constituents. However three major compounds were identified, namely Sulfurous acid, 2-ethylhexyl tridecyl ester, Phenol, 2,4-bis (1,1-dimethylethyl), and Trans-2-methyl-4-n-pentylthiane, S, S-Dioxide.
Hence the present study justifies the overwhelming circumstantial evidence as the most bioactive metabolites from the marine origin, which has potential utilization in pharmaceutical industry.
The aim of this study was to explore the bioactive potential of marine Streptomyces sp. isolated from marine soil and understand the bioactive properties of the crude extracts. It is clearly evident from the study that the bioactive metabolites produced by Streptomyces sp. exhibited good antibacterial, antioxidant and anticancer activity. Our results indicated that Starch casein medium was the good base for bioactive metabolite production. The taxonomic position of Streptomyces sp. isolated revealed unique pattern of phenotypic properties that distinguished it from representatives. The molecular characterization results provided valuable data for establishing the internal taxonomic structure of the genus. Hence high mortality rates, serious side effects, deficiencies of the available chemotherapeutics, and high costs during treatment clearly underscore the need to develop new anticancer agents, With the above significant features the strain could be recommended for its use in medicinal and agricultural sectors, an extensive knowledge on the behavior of natural compounds can be gained for the benefit of health.
最近，由于商业治疗药物的滥用，许多病原体产生了耐药性。
本研究的主要目的是评估壮观链霉菌VITJS10粗提物的生物活性潜力。
对壮观链霉菌VITJS10乙酸乙酯提取物进行抗菌、抗氧化和细胞毒性特性测试。使用16S r-DNA部分基因扩增和测序进行基因型鉴定。通过气相色谱-质谱联用仪（GC-MS）确定粗化学组成的真实性。
抗菌潜力显示，在20 mg/mL浓度下，该提取物对福氏志贺菌（MTCC编号：1457）（抑菌圈直径22 mm）、伤寒沙门氏菌（MTCC编号：1167）（23 mm）、大肠埃希氏菌（MTCC编号：1588）（22 mm）、铜绿假单胞菌（MTCC编号：4676）（22 mm）具有有效活性。使用2,2-二苯基-1-苦基肼（DPPH）法测定提取物的清除能力，结果显示在5 mg/mL浓度下其抑制率为95%。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法，利用肝癌细胞（HepG2）细胞系评估细胞毒性。提取物在IC50为250 μg/mL时显示出最大抑制作用，细胞存活率为53.6%。观察到16S rRNA基因序列与新菌株壮观链霉菌S3-1的最高同源性为99%。检测到VITJS10粗提物的化学成分有37种。然而，鉴定出了三种主要化合物，即亚硫酸2-乙基己基十三烷基酯、2,4-双（1,1-二甲基乙基）苯酚和反式-2-甲基-4-正戊基噻吩S,S-二氧化物。
因此，本研究证明了大量间接证据表明该海洋来源的代谢产物具有最强的生物活性，在制药工业中具有潜在的应用价值。
本研究的目的是探索从海洋土壤中分离出的海洋链霉菌的生物活性潜力，并了解粗提物的生物活性特性。研究清楚地表明，链霉菌产生的生物活性代谢产物具有良好的抗菌、抗氧化和抗癌活性。我们的结果表明，淀粉酪蛋白培养基是生物活性代谢产物产生的良好基础。分离出的链霉菌的分类地位显示出独特的表型特性模式，使其与其他代表菌株区分开来。分子鉴定结果为建立该属的内部分类结构提供了有价值的数据。因此，高死亡率、严重的副作用、现有化疗药物的缺陷以及治疗期间的高成本清楚地凸显了开发新抗癌药物的必要性。鉴于上述显著特征，该菌株可推荐用于医药和农业领域，可为健康受益获取关于天然化合物行为的广泛知识。
