Funaki Toshitaka, Yasuhara Tsutomu, Kugawa Satoshi, Yamazaki Yohei, Sugano Emi, Nagakura Yoshimi, Yoshida Katsuhiko, Fukuchi Kunihiko
Showa University School of Medicine, Department of Pathology, Japan.
Showa University Fujigaoka Hospital, Department of Respiratory Medicine, Japan.
Heliyon. 2019 Mar 28;5(3):e01415. doi: 10.1016/j.heliyon.2019.e01415. eCollection 2019 Mar.
The epidemiology of Panton-Valentine leukocidin (PVL)-positive MRSA in community-acquired methicillin-resistant (CA-MRSA) was examined. Three hundred and forty-two CA-MRSA strains that were susceptible to imipenem and cefazolin were isolated from 1107 samples (intravenous catheter, blood, sputum, urine, skin, wound, and pharynx) from outpatients at Showa University Hospital in Japan between September 2009 and March 2017. The PVL gene was detected in 46 of 342 CA-MRSA strains, accounting for 13.5%. The type of SCC was determined by detection of each SCC-specific region, class complex, and . SCC type IV comprised 33 strains, type V comprised 5 strains, type VII comprised 4 strains, and the unclassified type comprised 4 strains. Among the type IV strains, subtype IVa was dominant, comprising 23 of 33 strains, and the remaining 10 strains were of varying subtypes. The SCC type III-specific region, CZ049, was amplified in 2 type V strains, 4 type VII strains, and 4 unclassified strains. In 4 unclassified strains, CZ049 and were detected, but neither the SCC-specific region nor class complex was detected. The PVL-positive rate was lower than that in Western countries. The SCC types of PVL-positive CA-MRSA strains were found to vary, indicating a diverse spreading route.
对社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）中杀白细胞素（PVL）阳性的耐甲氧西林金黄色葡萄球菌（MRSA）的流行病学进行了研究。2009年9月至2017年3月期间，从日本昭和大学医院门诊患者的1107份样本（静脉导管、血液、痰液、尿液、皮肤、伤口和咽部）中分离出342株对亚胺培南和头孢唑林敏感的CA-MRSA菌株。在342株CA-MRSA菌株中，有46株检测到PVL基因，占13.5%。通过检测每个SCC特异性区域、类复合物等来确定SCC的类型。IV型SCC包括33株，V型包括5株，VII型包括4株，未分类类型包括4株。在IV型菌株中，IVa亚型占主导地位，33株中有23株，其余10株为不同亚型。在2株V型菌株、4株VII型菌株和4株未分类菌株中扩增出III型SCC特异性区域CZ049。在4株未分类菌株中，检测到CZ049和 ，但未检测到SCC特异性区域和类复合物。PVL阳性率低于西方国家。发现PVL阳性CA-MRSA菌株的SCC类型各不相同，表明传播途径多样。
