Muñoz William A, Trainor Paul A
Stowers Institute for Medical Research, Kansas City, MO, USA.
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, USA.
Methods Mol Biol. 2019;1976:107-119. doi: 10.1007/978-1-4939-9412-0_9.
Mammalian development occurs in utero, which makes it difficult to study the diverse morphogenetic events of neural crest cell development in vivo. Analyses of fixed samples in conjunction with histological methods to evaluate the spatiotemporal roles of specific genes of interest only provide snapshots of mammalian neural crest cell development. This chapter describes methods for isolating and culturing mouse embryos and their organs in vitro, outside the mother, to facilitate real-time imaging and functional analyses of the dynamics of neural crest cell development.
哺乳动物的发育在子宫内进行，这使得在体内研究神经嵴细胞发育过程中各种形态发生事件变得困难。结合组织学方法对固定样本进行分析，以评估特定感兴趣基因的时空作用，只能提供哺乳动物神经嵴细胞发育的瞬间图像。本章介绍了在体外、母体外分离和培养小鼠胚胎及其器官的方法，以促进对神经嵴细胞发育动态的实时成像和功能分析。
