Korsrud Gary O, Macneil James D
Agriculture Canada, Animal Pathology Laboratory, 116 Veterinary Road, Saskatoon, Saskatchewan S7N 2R3, Canada.
J Food Prot. 1988 Jan;51(1):43-46. doi: 10.4315/0362-028X-51.1.43.
The sensitivities of the Swab Test on Premises (STOP), the Calf Antibiotic and Sulfa Test (CAST) and a laboratory Microbial Inhibitor Test (MIT) previously used for confirmation of STOP results were compared. Different media were compared for the STOP and MIT. The CAST was more sensitive than the STOP for the 22 antibiotics tested. The sensitivity of the STOP procedure increased for 15 of the 22 antibiotics tested when the standard STOP medium, Antibiotic Medium No. 5 (AM-5), was replaced with Saskatoon Antibiotic Medium-3 (SAM-3), the medium used for thin-layer bioautography. When Antibiotic Medium No. 2 (AM-2) was used in the STOP procedure, the overall sensitivity was intermediate between that obtained using AM-5 and SAM-3. For the MIT, Micrococcus luteus ATCC 9341 provided greater sensitivity as a test organism than Bacillus cereus ATCC 11778 for 16 antibiotics and was less sensitive for 4 antibiotics, particularly chlortetracycline, oxytetracycline and tetracycline. M. luteus ATCC 9341 was notably more sensitive as a test organism for bacitracin and lincomycin than was B. cereus ATCC 11778.
比较了之前用于确认棉签现场检测（STOP）结果的现场棉签检测（STOP）、犊牛抗生素和磺胺检测（CAST）以及实验室微生物抑制剂检测（MIT）的灵敏度。对STOP和MIT使用的不同培养基进行了比较。对于所检测的22种抗生素，CAST比STOP更灵敏。当标准的STOP培养基5号抗生素培养基（AM - 5）被用于薄层生物自显影的萨斯卡通抗生素培养基 - 3（SAM - 3）取代时，所检测的22种抗生素中有15种的STOP检测程序灵敏度提高。当在STOP检测程序中使用2号抗生素培养基（AM - 2）时，总体灵敏度介于使用AM - 5和SAM - 3时获得的灵敏度之间。对于MIT，作为测试菌株，藤黄微球菌ATCC 9341对16种抗生素的灵敏度高于蜡样芽孢杆菌ATCC 11778，而对4种抗生素（特别是金霉素、土霉素和四环素）的灵敏度较低。作为杆菌肽和林可霉素的测试菌株，藤黄微球菌ATCC 9341的灵敏度明显高于蜡样芽孢杆菌ATCC 11778。
