Read R B, Bradshaw J G, Swartzentruber A A, Brazis A R
Division of Microbiology, Food and Drug Administration, Cincinnati, Ohio 45226.
Appl Microbiol. 1971 May;21(5):806-8. doi: 10.1128/am.21.5.806-808.1971.
A disc assay method for testing sulfa drugs and antibiotics in milk was developed wherein Bacillus megaterium ATCC 9855 was used as the test organism and Mueller-Hinton agar was used as the test substrate. Incubation was at 37 C for 4 to 5 hr. The test procedure is an improvement over the Bacillus subtilis-Antibiotic Medium No. 1 method, as described in Standard Methods for the Examination of Dairy Products, in that it is sensitive to eight sulfa drugs and to bacitracin without a significant change in sensitivity to eight other antibiotics commonly used for mastitis therapy.
开发了一种用于检测牛奶中磺胺类药物和抗生素的纸片扩散法，其中巨大芽孢杆菌ATCC 9855用作测试微生物，穆勒-欣顿琼脂用作测试底物。在37℃下孵育4至5小时。该测试方法是对《乳制品检验标准方法》中所述的枯草芽孢杆菌-抗生素1号培养基方法的改进，因为它对八种磺胺类药物和杆菌肽敏感，而对常用于乳腺炎治疗的其他八种抗生素的敏感性没有显著变化。
