Carrera de Medicina, Facultad de Ciencias de la Salud, Universidad de las Américas, Quito, Ecuador; Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencias a los Antibióticos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador; Life Science Initiative, lsi-ec.com, Quito, Ecuador.
Life Science Initiative, lsi-ec.com, Quito, Ecuador; Laboratorio Clínico e Inmunológico INMUNOLAB, Quito, Ecuador.
J Glob Antimicrob Resist. 2019 Sep;18:263-268. doi: 10.1016/j.jgar.2019.04.002. Epub 2019 Apr 10.
This study focused on estimating the prevalence of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, carbapenemases and MCR-1-producing Escherichia coli in canine faeces from a public park in Quito, Ecuador.
Phenotypic and genotypic characterisation of E. coli isolated from 50 canine faecal samples recovered from a city park in Quito was performed. In addition, a multiple choice survey was conducted among 50 dog owners.
Of the 50 faecal samples, 20 (40.0%) presented E. coli resistant to ceftriaxone. Moreover, 23 E. coli isolates were recovered for further analysis. All of the isolates showed as multidrug-resistant (MDR) phenotype (resistant to three or more antibiotic families). Resistance to carbapenems, tigecycline and amikacin was not observed. No major clonal relatedness was observed among the resistant isolates. The ESBL genes bla, bla and bla were the most common. Two isolates harboured the bla gene and one isolate harboured both mcr-1 and bla Statistical analysis showed that older people were more conscious of collecting and disposing of dog faeces than subjects aged <35 years (P < 0.05).
The finding of MDR E. coli in dog faeces in a city park in Ecuador illustrates the importance of analysing canine faeces in public settings (e.g. parks, playgrounds) as part of surveillance programmes for MDR E. coli. In addition, this research might be a sentinel sampling method to gain a better understanding of community sources of antibiotic-resistant Enterobacteriaceae at human-animal-environment interfaces.
本研究旨在估算厄瓜多尔基多市一个公园犬粪便中产超广谱β-内酰胺酶（ESBLs）、质粒介导的AmpC β-内酰胺酶、碳青霉烯酶和 MCR-1 大肠杆菌的流行率。
对从基多市一个公园采集的 50 份犬粪便样本中分离的大肠杆菌进行表型和基因型特征分析。此外，还对 50 名犬主人进行了多项选择调查。
在 50 份粪便样本中，有 20 份（40.0%）对头孢曲松表现出耐药性。此外，还回收了 23 株大肠杆菌进行进一步分析。所有分离株均表现出多药耐药（MDR）表型（对三种或三种以上抗生素家族耐药）。未观察到对碳青霉烯类、替加环素和阿米卡星的耐药性。耐药分离株之间未观察到主要的克隆相关性。最常见的 ESBL 基因是 blaCTX-M、blaTEM 和 blaSHV。有 2 株分离株携带 blaCTX-M 基因，1 株分离株携带 mcr-1 和 blaCTX-M 基因。统计分析表明，老年人比<35 岁的人更有意识地收集和处理犬粪便（P<0.05）。
在厄瓜多尔基多市一个城市公园的犬粪便中发现 MDR 大肠杆菌，说明分析公共环境（如公园、游乐场）中犬粪便作为 MDR 大肠杆菌监测计划的一部分的重要性。此外，这项研究可能是一种监测方法，可以更好地了解人-动物-环境界面中抗生素耐药肠杆菌科的社区来源。
