Wang Yanfen, Zhao Hang, Cao Jiayuan, Yin Xinming, Guo Yashuang, Guo Lihua, Wu Haiyan, Zhang Meng
School of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Viruses. 2022 Feb 6;14(2):331. doi: 10.3390/v14020331.
is, globally, one of the most economically important phytopathogenic fungi worldwide, causing the canker and dieback of fruit trees. An increasing number of viruses infecting have lately been reported, several of which could confer hypovirulence. In this study, isolated from strain ZM170285-1 of , a novel double-stranded RNA (dsRNA) mycovirus, tentatively named Botryosphaeria dothidea partitivirus 2 (BdPV2), was identified well. The BdPV2 harbored three dsRNA segments (1-3) with lengths of 1751, 1568, and 1198 bp, which encoded an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), and a hypothetical protein of unknown function, respectively. BLASTp searches revealed that the predicted protein sequences of dsRNA1 and dsRNA2 had the highest identities (74.95% and 61.01%) with the corresponding dsRNAs of Penicillium stoloniferum virus S (PsV-S), whereas dsRNA3 shared the highest identity (32.95%) with the dsRNA3 of Aspergillus ochraceous virus 1 (AoV1). Phylogenetic analysis indicated that BdPV2 belonged to the genus and family. To our knowledge, this is the first report of a in .
在全球范围内，是世界上经济上最重要的植物病原真菌之一，可导致果树溃疡和枯死。最近报道了越来越多感染的病毒，其中几种可导致低毒力。在本研究中，从的ZM170285-1菌株中分离出一种新型双链RNA（dsRNA）真菌病毒，暂命名为葡萄座腔菌分病毒2（BdPV2），并对其进行了很好的鉴定。BdPV2含有三个dsRNA片段（1-3），长度分别为1751、1568和1198 bp，分别编码一种RNA依赖的RNA聚合酶（RdRp）、一种衣壳蛋白（CP）和一种功能未知的假定蛋白。BLASTp搜索显示，dsRNA1和dsRNA2的预测蛋白序列与匍枝根霉病毒S（PsV-S）的相应dsRNAs具有最高的同一性（74.95%和61.01%），而dsRNA3与赭曲霉病毒1（AoV1）的dsRNA3具有最高的同一性（32.95%）。系统发育分析表明，BdPV2属于属和科。据我们所知，这是中关于的首次报道。
